数量	カタログ番号	注文内容	Qty/Pk	価格
			96-Well Plate

数量	カタログ番号	注文内容	Qty/Pk	価格
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

AP1140 Sigma-AldrichAnti-GBA Mouse mAb (2E2)

Anti-GBA, mouse monoclonal, clone 2E2, recognizes the ~60 kDa GBA in MCF-7 and HeLa cells and human breast cancer tissue. It is validated for use in ELISA, WB, ICC, and IHC on paraffin sections.

Anti-GBA Mouse mAb (2E2): のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

(M)SDS
試験成績書（CoA）
参考資料
Data Sheet

同義語: Anti-Glucosidase β, Acid

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概要

Replacement Information

主要スペック表

Species Reactivity	Host	Antibody Type
H	M	Monoclonal Antibody

価格＆在庫状況

カタログ番号	在庫状況	包装	Qty/Pk	価格	数量
AP1140-100UGCN	在庫状況検索中… — 現在国内在庫なし現在国内在庫有り販売中止在庫僅少現在国内在庫あり在庫を照会するにはログインしてください現在国内在庫有り現在国内在庫なし現在国内在庫有り Remaining : Will advise Remaining : Will advise 注文対象外お問合せください Contact Customer Service お問合せくださいお問合せください		100 μg	価格を検索中… 価格が見つかりません Minimum Quantity needs to be mulitiple of Maximum Quantity is 弊社照会詳細を表示　値引 () — 弊社照会納入価格を閲覧するにはログインしてください	在庫状況を照会 —	お気に入り/ショッピングリストに追加お気に入り/ショッピングリストに追加お気に入りに追加されました

Description
Overview	Recognizes the ~60 kDa GBA protein in MCF-7 and HeLa cells and human breast cancer tissue.
Catalogue Number	AP1140
Brand Family	Calbiochem®
Synonyms	Anti-Glucosidase β, Acid
Application Data	Detection of human GBA by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Primary antibody: Anti-GBA Mouse mAb (2E2) (Cat. No. AP1140) (1-5 µg/ml). Detection: chemiluminescence. Detection of human GBA by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-GBA Mouse mAb (2E2) (Cat. No. AP1140) (10 µg/ml). Detection: fluorescence. Detection of human GBA by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human breast cancer tissue. Primary antibody: Anti-GBA Mouse mAb (2E2) (Cat. No. AP1140) (3 µg/ml). Detection: colorimetric (DAB).

References
References	Campeau, P.M., et al. 2009. Blood 114, 3181.

Product Information
Form	Liquid
Formulation	In PBS, pH 7.2.
Negative control	293T
Positive control	MCF-7 cells, HeLa cells, Human breast cancer tissue
Preservative	None
Quality Level	MQ100

Applications
Key Applications	Enzyme-Linked Immunosorbent Assay Immunoblotting (Western Blotting) Immunocytochemistry Paraffin Sections
Application Notes	ELISA (see comments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml) Paraffin Sections (3 µg/ml)
Application Comments	For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Biological Information
Immunogen	A recombinant polypeptide corresponding to amino acids of 146-236 human GBA, expressed as a GST fusion protein
Clone	2E2
Host	Mouse
Isotype	IgG_2a
Species Reactivity	Human
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Regulatory Review
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
カタログ番号	GTIN
AP1140-100UGCN	04055977227857

Documentation

Anti-GBA Mouse mAb (2E2) (M)SDS

タイトル
英語版製品安全データシート（(M)SDS）

Anti-GBA Mouse mAb (2E2) 試験成績書（CoA）

タイトル	ロット番号
AP1140	ロット番号を入力

参考資料

参考資料の概要
Campeau, P.M., et al. 2009. Blood 114, 3181.

データシート

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	18-October-2010 RFH
Synonyms	Anti-Glucosidase β, Acid
Application	ELISA (see comments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml) Paraffin Sections (3 µg/ml)
Application Data	Detection of human GBA by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Primary antibody: Anti-GBA Mouse mAb (2E2) (Cat. No. AP1140) (1-5 µg/ml). Detection: chemiluminescence. Detection of human GBA by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-GBA Mouse mAb (2E2) (Cat. No. AP1140) (10 µg/ml). Detection: fluorescence. Detection of human GBA by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human breast cancer tissue. Primary antibody: Anti-GBA Mouse mAb (2E2) (Cat. No. AP1140) (3 µg/ml). Detection: colorimetric (DAB).
Description	Purified mouse monoclonal antibody. Recognizes the ~60 kDa GBA protein.
Background	Acid beta-glucosidase (GBA) is a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. It catalyzes the breakdown of the glycolipid glucosylceramide to ceramide and glucose. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides.
Host	Mouse
Immunogen	A recombinant polypeptide corresponding to amino acids of 146-236 human GBA, expressed as a GST fusion protein
Clone	2E2
Isotype	IgG_2a
Species	human
Positive control	MCF-7 cells, HeLa cells, Human breast cancer tissue
Negative control	293T
Form	Liquid
Formulation	In PBS, pH 7.2.
Preservative	None
Comments	For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Regulatory Review
References	Campeau, P.M., et al. 2009. Blood 114, 3181.

カテゴリー

Life Science Research > Antibodies and Assays > Primary Antibodies

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