数量	カタログ番号	注文内容	Qty/Pk	価格
			96-Well Plate

数量	カタログ番号	注文内容	Qty/Pk	価格
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

GR01L Sigma-AldrichAnti-EGFR (Ab-1) Mouse mAb (528)

Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studies.

Anti-EGFR (Ab-1) Mouse mAb (528): のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

(M)SDS
試験成績書（CoA）
参考資料
Data Sheet

同義語: Anti-Epidermal Growth Factor Receptor

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概要

Replacement Information

主要スペック表

Species Reactivity	Host	Antibody Type
H	M	Monoclonal Antibody

価格＆在庫状況

カタログ番号	在庫状況	包装	Qty/Pk	価格	数量
GR01L-100UGCN	在庫状況検索中… — 現在国内在庫なし現在国内在庫有り販売中止在庫僅少現在国内在庫あり在庫を照会するにはログインしてください現在国内在庫有り現在国内在庫なし現在国内在庫有り Remaining : Will advise Remaining : Will advise 注文対象外お問合せください Contact Customer Service お問合せくださいお問合せください	樹脂ｱﾝﾌﾟﾙ	100 μg	価格を検索中… 価格が見つかりません Minimum Quantity needs to be mulitiple of Maximum Quantity is 弊社照会詳細を表示　値引 () — 弊社照会納入価格を閲覧するにはログインしてください	在庫状況を照会 —	お気に入り/ショッピングリストに追加お気に入り/ショッピングリストに追加お気に入りに追加されました

Description
Overview	Recognizes the EGF receptor protein in A431 cells and normal skin.
Catalogue Number	GR01L
Brand Family	Calbiochem®
Synonyms	Anti-Epidermal Growth Factor Receptor

References
References	Hunter T. 1984. Nature 311, 414. Masui, H., et al. 1984. Cancer Res. 44, 1002. Reynolds, F. H., Jr., et al. 1981. Nature 292, 259. Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.

Product Information
Form	Lyophilized
Formulation	Lyophilized from 20 mM ammonium bicarbonate solution, 0.1 mg FAF-BSA.
Positive control	A-431 cells or skin tissue
Preservative	None
Quality Level	MQ100

Applications
Application References	Original Clone Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA 80, 1337. Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7761. Kawamoto, T., et al. 1984. J. Biol Chem. 259, 7761. Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Molec. Biol. Med. 1, 511. Paraffin Sections Burton B.C., et al. 2002. Clin. Cancer Res. 1, 180. Mishima, K., et al. 2001. Cancer Res. 61, 7703. Pilcher B.K., et al. 1999. J. Biol. Chem. 274, 10372.
Key Applications	Flow Cytometry Frozen Sections Immunofluorescence Immunoprecipitation Neutralization Studies Paraffin Sections
Application Notes	Flow Cytometry (5 µg/ml) Frozen Sections (2.5 µg/ml) Immunofluorescence (5 µg/ml) Immunoprecipitation (1 µg/sample) Neutralization Studies (see comments and application references) Paraffin Sections (see application references)
Application Comments	This antibody binds to A431, HeLa, and other human cells but not to rodent cells. This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	partially purified, human EGFR from human A431 cells
Immunogen	Human
Clone	528
Host	Mouse
Isotype	IgG_2a
Species Reactivity	Human
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Ok to freeze
Special Instructions	We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
カタログ番号	GTIN
GR01L-100UGCN	04055977220155

Documentation

Anti-EGFR (Ab-1) Mouse mAb (528) (M)SDS

タイトル
英語版製品安全データシート（(M)SDS）

Anti-EGFR (Ab-1) Mouse mAb (528) 試験成績書（CoA）

タイトル	ロット番号
GR01L	ロット番号を入力

参考資料

参考資料の概要
Hunter T. 1984. Nature 311, 414. Masui, H., et al. 1984. Cancer Res. 44, 1002. Reynolds, F. H., Jr., et al. 1981. Nature 292, 259. Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.

データシート

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	25-October-2007 JSW
Synonyms	Anti-Epidermal Growth Factor Receptor
Application	Flow Cytometry (5 µg/ml) Frozen Sections (2.5 µg/ml) Immunofluorescence (5 µg/ml) Immunoprecipitation (1 µg/sample) Neutralization Studies (see comments and application references) Paraffin Sections (see application references)
Description	Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa EGFR protein.
Background	Epidermal growth factor (EGF) initiates its effects on cell growth through interaction with a 170,000 dalton cell surface glycoprotein receptor. Binding of EGF or transforming growth factor a to the receptor activates a tyrosine-specific protein kinase intrinsic to the EGF-receptor protein. The phosphotyrosine content of target cells increases rapidly and the EGF-receptor is phosphorylated on tyrosine residues.
Host	Mouse
Immunogen species	Human
Immunogen	partially purified, human EGFR from human A431 cells
Clone	528
Isotype	IgG_2a
Species	human, not mouse, not rat
Positive control	A-431 cells or skin tissue
Form	Lyophilized
Formulation	Lyophilized from 20 mM ammonium bicarbonate solution, 0.1 mg FAF-BSA.
Preservative	None
Comments	This antibody binds to A431, HeLa, and other human cells but not to rodent cells. This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.
Toxicity	Standard Handling
References	Hunter T. 1984. Nature 311, 414. Masui, H., et al. 1984. Cancer Res. 44, 1002. Reynolds, F. H., Jr., et al. 1981. Nature 292, 259. Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
Application references	Original Clone Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA 80, 1337. Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7761. Kawamoto, T., et al. 1984. J. Biol Chem. 259, 7761. Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Molec. Biol. Med. 1, 511. Paraffin Sections Burton B.C., et al. 2002. Clin. Cancer Res. 1, 180. Mishima, K., et al. 2001. Cancer Res. 61, 7703. Pilcher B.K., et al. 1999. J. Biol. Chem. 274, 10372.

Related Products

カタログ番号

説明

GR01

Anti-EGFR (Ab-1) Mouse mAb (528)

価格＆在庫状況を表示

カテゴリー

Life Science Research > Antibodies and Assays > Primary Antibodies

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