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AB4330 Anti-DDX4 (Vasa) Antibody

Anti-DDX4 (Vasa) Antibody detects level of DDX4 (Vasa) & has been published & validated for use in WB, IH(P).

Anti-DDX4 (Vasa) Antibody: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
Research Category: Stem Cell Research Research Sub-Category: Pluripotent & Early Differentiation, RNA Metabolism & Binding Proteins Gene Symbol: VASA, MVH, DDX4 UniProt Number: Q61496
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AB4330
100 µL  
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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB4330
      DescriptionAnti-DDX4 (Vasa) Antibody
      Alternate Names
      • DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
      • probable ATP-dependent RNA helicase DDX4
      • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4
      • Vasa homolog
      • DEAD box protein 4
      Background InformationDDX4 (Vasa or MVH) is a member of the DEAD family of proteins and is a probable ATP-dependent RNA helicase. DDX4 (Vasa) proteins are mostly localized in testicular germ cells in the cytoplasm of spermatocytes. DDX4 (Vasa) is a high-copy extragenic suppressor gene. DDX4 (Vasa) is shown to be significantly decreased in spermatozoa of men that are infertile and may have an important role in male fertility.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Mouse testis tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-DDX4 (Vasa) Antibody detects level of DDX4 (Vasa) & has been published & validated for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected DDX4 (Vasa) in normal human testis tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of mouse DDX4 (Vasa).
      EpitopeC-terminus
      HostRabbit
      SpecificityThis antibody recognizes DDX4 (Vasa) at the C-terminus.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • VASA
      • MVH
      • DDX4
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: May play a role in germ cell development.

      CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate.

      SUBUNIT STRUCTURE: Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. N-terminus interacts with RANBP9. Interacts with PIWIL2 and MAEL. Ref.3 SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm › perinuclear region.

      TISSUE SPECIFICTY: Testis.

      DEVELOPMENTAL STAGE: Expressed in spermatogenic cells from the spermatocyte stage to the round spermatid stage.

      SEQUENCE SIMILARITIES: Belongs to the DEAD box helicase family. DDX4/VASA subfamily.

      Contains 1 helicase ATP-binding domain.

      Contains 1 helicase C-terminal domain.
      Molecular Weight~76 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse testis tissue lysate.

      Western Blot Analysis: A 1:500 dilution of this antibody detected DDX4 (Vasa) in 10 µg of mouse testis tissue lysate. Western Blot performed without Tween.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      AB4330 04053252526923

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      カテゴリー

      Life Science Research > Antibodies and Assays > Primary Antibodies