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MABT520 Anti-Cytokeratin 18, clone 4D11E4 Antibody

Anti-Cytokeratin 18, clone 4D11E4 Antibody is an antibody against Cytokeratin 18 for use in Western Blotting, Immunocytochemistry, ELISA.

Anti-Cytokeratin 18, clone 4D11E4 Antibody: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: KRT18, CYK18, PIG46 UniProt Number: P05783
MABT520
100 µL  
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      概要

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABT520
      Replaces04-586
      DescriptionAnti-Cytokeratin 18, clone 4D11E4 Antibody
      Alternate Names
      • Keratin
      • type I cytoskeletal 18
      • Cell proliferation-inducing gene 46 protein
      • Cytokeratin-18
      • CK-18
      • Keratin-18
      • K18
      • Cytokeratin 18
      Background InformationKeratin, type I cytoskeletal 18 (KRT18), also known as Cytokeratin-18 (CK-18), are intermediate filament phosphoglycoproteins that are expressed preferentially in glandular epithelia. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Together with actin microfilaments and microtubules, keratin filaments make up the cytoskeletons of vertebrate epithelial cells, forming alpha-helical coiled-coil dimers which associate laterally and end-to-end to form 10-nm diameter filaments.
      References
      Product Information
      FormatAscites
      PresentationMouse monoclonal IgG2bascitic fluid containing 0.03% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationAnti-Cytokeratin 18, clone 4D11E4 Antibody is an antibody against Cytokeratin 18 for use in Western Blotting, Immunocytochemistry, ELISA.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected Cytokeratin 18 in HeLa, NIH/3T3, A549, Jurkat, MCF-7, HepG2, A431, and K562 cell lysates.

      Immunohistochemistry Analysis (IHC): A 1:200-1,000 dilution from a representative lot detected Cytokeratin 18 in human breast carcinoma, hepatocarcinoma, stomach cancer, and colon cancer tissues.
      Biological Information
      ImmunogenPurified recombinant fragment of human Cytokeratin 18 expressed in E. Coli. 
      Clone4D11E4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • KRT18
      • CYK18
      • PIG46
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~62 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting using cell lysates.

      Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected Cytokeratin 18 in HeLa, NIH/3T3, A549, Jurkat, MCF-7, HepG2, A431, and K562 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      MABT520 04055977171501

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      カテゴリー

      Life Science Research > Antibodies and Assays > Primary Antibodies