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MABC1768-25UG Anti-CDC73 Antibody, clone 2H1-F10-B9

MABC1768-25UG
25 μg  
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      概要

      Replacement Information
      Description
      Catalogue NumberMABC1768-25UG
      DescriptionAnti-CDC73 Antibody, clone 2H1-F10-B9
      Alternate Names
      • Parafibromin
      • Cell division cycle protein 73 homolog
      • Hyperparathyroidism 2 protein
      Background InformationParafibromin (UniProt: Q6P1J9; also known as Cell division cycle protein 73 homolog, Hyperparathyroidism 2 protein, CDC73) is encoded by the CDC73 (also known as C1orf28, HRPT2) gene (Gene ID: 79577) in human. CDC73 is a tumor suppressor that is involved in transcriptional and post-transcriptional control pathways. It is expressed in adrenal and parathyroid glands and in kidney and heart. It is a component of the PAF1 complex with multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1 complex associates with RNA polymerase II through the interaction with C-terminal domain. CDC73 is also reported to physically associates with the cleavage and polyadenylation specificity factor (CPSF) and cleavage stimulation factor (CstF) complexes that are required for the maturation of mRNA 3 ends in the cell nucleus. Mutations in CDC73 are reported to cause parathyroid carcinoma and hyperparathyroidism and the loss of CDC73 immunoreactivity is considered as a distinguishing feature of parathyroid carcinoma. (Ref.: Tan, MH et al., (2004). Clin. Cancer Res. 10; 6629-6637; Rozenblatt-Rosen, O. et al. (2009). Proc. Natl. Acad. Sci. USA. 106(3); 755-760).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationThis mouse monoclonal Anti-CDC73, clone 2H1-F10-B9, Cat. No. MABC1768, is a mouse monoclonal antibody that detects Parafibromin (CDC73) and is tested for use in Immunocytochemistry, Immunohistochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A representative lot detected CDC73 in Immunocytochemistry applications (Tan, M.H., et. al. (2004). Clin Cancer Res. 10(19):6629-37).

      Immunohistochemistry Analysis: A representative lot detected CDC73 in Immunohistochemistry applications (Tan, M.H., et. al. (2004). Clin Cancer Res. 10(19):6629-37).

      Western Blotting Analysis: A representative lot detected CDC73 in Western Blotting applications (Tan, M.H., et. al. (2004). Clin Cancer Res. 10(19):6629-37).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 13 amino acids from the N-terminal region of human CDC73 (Parafibromin).
      EpitopeN-terminal
      Clone2H1-F10-B9
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 2H1-F10-B9 is a mouse monoclonal antibody that detects CDC73 (Parafibromin). It targets an epitope within 13 amino acids from the N-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Monkey, Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CDC73
      • C1orf28
      • HRPT2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~60 kDa observed; 60.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected CDC73 in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      MABC1768-25UG 04061842626090

      Documentation

      Anti-CDC73 Antibody, clone 2H1-F10-B9 (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      Anti-CDC73 Antibody, clone 2H1-F10-B9 試験成績書(CoA)

      タイトルロット番号
      Anti-CDC73, clone 2H1-F10-B9 - Q3503513 Q3503513

      参考資料

      参考資料の概要Pub Med ID
      Loss of parafibromin immunoreactivity is a distinguishing feature of parathyroid carcinoma
      Min-Han Tan 1 , Carl Morrison, Pengfei Wang, Ximing Yang, Carola J Haven, Chun Zhang, Ping Zhao, Maria S Tretiakova, Eeva Korpi-Hyovalti, John R Burgess, Khee Chee Soo, Wei-Keat Cheah, Brian Cao, James Resau, Hans Morreau, Bin Tean The
      Clin Cancer Res  10(19)  6629-37  2004

      概要を表示する
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