Millipore Sigma Vibrant Logo

MABF396 Anti-CD25 Antibody (mouse), APC, clone PC61.5

This rat monoclonal antibody Anti-CD25 (mouse), APC & clone PC61.5 validated for use in IP;IHC;Flow Cytometry for the detection of CD25.

Anti-CD25 Antibody (mouse), APC, clone PC61.5: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
Research Category: Inflammation & Immunology Gene Symbol: Il2ra, Il2r UniProt Number: P01590
View This Product on Sigma-Aldrich
MABF396
100 µg  
価格を検索中…
価格が見つかりません
Minimum Quantity needs to be mulitiple of
Maximum Quantity is
弊社照会 詳細を表示　
値引
()
 
—
弊社照会
—
現在国内在庫なし 現在国内在庫なし
現在国内在庫有り 
販売中止
在庫僅少
現在国内在庫あり
    Remaining : Will advise
      Remaining : Will advise
      注文対象外
      お問合せください
      Contact Customer Service

      Special Offers［海外情報］

       

      お問合せください

      概要

      Replacement Information

      Special Offers［海外情報］

      100% Performance Guaranteed

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MIP, IHC, FCRAPCMonoclonal Antibody
      Description
      Catalogue NumberMABF396
      DescriptionAnti-CD25 Antibody (mouse), APC, clone PC61.5
      Alternate Names
      • Interleukin-2 receptor subunit alpha
      • IL-2 receptor subunit alpha
      • IL-2-RA
      • IL-2R subunit alpha
      • IL2-RA
      • p55
      • CD25
      Background InformationThe PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells. The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used for depletion of Treg cells in vivo, as well as to distinguish Treg cells from naïve or conventional T cells which are CD25-.
      References
      Product Information
      FormatAPC
      PresentationPurified rat monoclonal antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis rat monoclonal antibody Anti-CD25 (mouse), APC & clone PC61.5 validated for use in IP;IHC;Flow Cytometry for the detection of CD25.
      Key Applications
      • Immunoprecipitation
      • Immunohistochemistry
      • Flow Cytometry
      Biological Information
      ImmunogenImmunogen information for this antibody is proprietary information.
      ClonePC61.5
      Concentration0.2 mg/mL
      HostRat
      IsotypeIgG1λ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Il2ra
      • Il2r
      Purification MethodSize Exclusion Chromatography
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceThe monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

      Flow Cytometry Analysis: 0.125 µg of this antibody detected CD25 in one million C57BI/6 bone marrow cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsIt is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      MABF396 04053252959882

      関連製品＆アプリケーション

      Related Products

      カタログ番号 説明  
      MABF397 Anti-CD25 Antibody (mouse), PE-Cy7, clone PC61.5 価格＆在庫状況を表示
      MABF548 Anti-CD25 Antibody (mouse), APC, clone PC61.5 価格＆在庫状況を表示
      MABF549 Anti-CD25 Antibody (mouse), PE-Cy7, clone PC61.5 価格＆在庫状況を表示
      MABF550 Anti-CD25 Antibody (mouse), PerCP-Cy5.5, clone PC61.5 価格＆在庫状況を表示

      カテゴリー

      Life Science Research > Antibodies and Assays > Primary Antibodies