数量	カタログ番号	注文内容	Qty/Pk	価格
			96-Well Plate

数量	カタログ番号	注文内容	Qty/Pk	価格
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

217660 Sigma-AldrichAnti-CD18 Mouse mAb (IB4)

Anti-CD18, mouse monoclonal, clone IB4, recognizes the ~95 kDa β2 integrin subunit. It is validated for FC, IP, IHC on frozen sections, and cell inhibition assays.

Anti-CD18 Mouse mAb (IB4): のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

(M)SDS
試験成績書（CoA）
参考資料
Data Sheet

同義語: Anti-β2 Integrin

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概要

Replacement Information

主要スペック表

Species Reactivity	Host	Antibody Type
H	M	Monoclonal Antibody

価格＆在庫状況

カタログ番号	在庫状況	包装	Qty/Pk	価格	数量
217660-100UGCN	在庫状況検索中… — 現在国内在庫なし現在国内在庫有り販売中止在庫僅少現在国内在庫あり在庫を照会するにはログインしてください現在国内在庫有り現在国内在庫なし現在国内在庫有り Remaining : Will advise Remaining : Will advise 注文対象外お問合せください Contact Customer Service お問合せくださいお問合せください	樹脂ｱﾝﾌﾟﾙ	100 μg	価格を検索中… 価格が見つかりません Minimum Quantity needs to be mulitiple of Maximum Quantity is 弊社照会詳細を表示　値引 () — 弊社照会納入価格を閲覧するにはログインしてください	在庫状況を照会 —	お気に入り/ショッピングリストに追加お気に入り/ショッピングリストに追加お気に入りに追加されました

Description
Overview	Recognizes the ~95 kDa β2 integrin subunit. Blocks the binding of ICAM-1 and ICAM-3 to LFA-1.
Catalogue Number	217660
Brand Family	Calbiochem®
Synonyms	Anti-β2 Integrin

References
References	Berman, M.E., et al. 1996. J. Immunol. 156, 1515. Kaneko, M., et al. 1995. J. Immunol. 155, 2631. Mazzone, A., and Ricevuti, G. 1995. Haematologica 80, 161. Kaneko, M., et al. 1995. J. Immunol. 155, 2631. Landis, R.C., et al. 1994. J. Cell Biol. 126, 529. Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 5699.

Product Information
Form	Liquid
Formulation	In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Negative control	Nalm-6 cells
Positive control	HPB-MLT cells
Preservative	0.5 mg/ml gentamicin sulfate
Quality Level	MQ100

Applications
Key Applications	Cell Inhibition Assays Flow Cytometry Frozen Sections Immunoprecipitation
Application Notes	Flow Cytometry (5-10 µg/ml) Frozen Sections (see comments) Immunoprecipitation (see comments) Cell Inhibition Assays (see comments)
Application Comments	Blocks the binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x10⁵ cultured human HPB-MLT cells using anti-CD18 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for immunoprecipitation and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.

Biological Information
Immunogen	human peripheral blood monocytes
Immunogen	Human
Clone	IB4
Host	Mouse
Purity	≥95% by SDS-PAGE
Isotype	IgG_2a
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Ok to freeze
Special Instructions	Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
カタログ番号	GTIN
217660-100UGCN	04055977202946

Documentation

Anti-CD18 Mouse mAb (IB4) (M)SDS

タイトル
英語版製品安全データシート（(M)SDS）

Anti-CD18 Mouse mAb (IB4) 試験成績書（CoA）

タイトル	ロット番号
217660	ロット番号を入力

参考資料

参考資料の概要
Berman, M.E., et al. 1996. J. Immunol. 156, 1515. Kaneko, M., et al. 1995. J. Immunol. 155, 2631. Mazzone, A., and Ricevuti, G. 1995. Haematologica 80, 161. Kaneko, M., et al. 1995. J. Immunol. 155, 2631. Landis, R.C., et al. 1994. J. Cell Biol. 126, 529. Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 5699.

データシート

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	08-July-2009 JSW
Synonyms	Anti-β2 Integrin
Application	Flow Cytometry (5-10 µg/ml) Frozen Sections (see comments) Immunoprecipitation (see comments) Cell Inhibition Assays (see comments)
Description	Protein A purified mouse monoclonal antibody. Recognizes the ~95 kDa β2 integrin subunit (CD18).
Background	Human CD18 (β2 integrin) is expressed on leukocytes as part of a heterodimeric complex with either CD11a, b or c (integrins α^L, α^M, or α^X). CD18/CD11 heterodimers function in cell adhesion in immune and inflammatory responses.
Host	Mouse
Immunogen species	Human
Immunogen	human peripheral blood monocytes
Clone	IB4
Isotype	IgG_2a
Species	human
Positive control	HPB-MLT cells
Negative control	Nalm-6 cells
Form	Liquid
Formulation	In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Concentration Label	Please refer to vial label for lot-specific concentration
Purity	≥95% by SDS-PAGE
Preservative	0.5 mg/ml gentamicin sulfate
Comments	Blocks the binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x10⁵ cultured human HPB-MLT cells using anti-CD18 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for immunoprecipitation and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
Storage	+2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Toxicity	Standard Handling
References	Berman, M.E., et al. 1996. J. Immunol. 156, 1515. Kaneko, M., et al. 1995. J. Immunol. 155, 2631. Mazzone, A., and Ricevuti, G. 1995. Haematologica 80, 161. Kaneko, M., et al. 1995. J. Immunol. 155, 2631. Landis, R.C., et al. 1994. J. Cell Biol. 126, 529. Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 5699.

カテゴリー

Life Science Research > Antibodies and Assays > Primary Antibodies

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