Millipore Sigma Vibrant Logo

ABC131 Anti-Ambra1 Antibody

Anti-Ambra1 Antibody is a rabbit polyclonal antibody, validated for use in western blotting.

Anti-Ambra1 Antibody: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: AMBRA1 UniProt Number: A2AH22
View This Product on Sigma-Aldrich
ABC131
100 µg  
価格を検索中…
価格が見つかりません
Minimum Quantity needs to be mulitiple of
Maximum Quantity is
弊社照会 詳細を表示　
値引
()
 
—
弊社照会
—
現在国内在庫なし 現在国内在庫なし
現在国内在庫有り 
販売中止
在庫僅少
現在国内在庫あり
    Remaining : Will advise
      Remaining : Will advise
      注文対象外
      お問合せください
      Contact Customer Service

      Special Offers［海外情報］

       

      お問合せください

      概要

      Replacement Information

      Special Offers［海外情報］

      100% Performance Guaranteed

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC131
      DescriptionAnti-Ambra1 Antibody
      Alternate Names
      • Activating molecule in BECN1-regulated autophagy protein 1
      • Ambra
      • Ambra-1
      Background InformationActivating molecule in BECN1-regulated autophagy protein 1 (AMBRA1) is a developmental protein that is involved in autophagy, differentiation and nuerogenesis. AMBRA1 regulates autophagy and development of the nervous system, normal cell survival and proliferation, and the control of protein turnover during neuronal development.
      References
      Product Information
      FormatAffinity Purified
      Control
      • MCF7 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Ambra1 Antibody is a rabbit polyclonal antibody, validated for use in western blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 0.5 µg/mL of a representative lot detected Ambra1 in 10 µg of HeLa cell lysate.
      Western Blotting Analysis: 1 µg/mL of a representative lot detected Ambra1 in human cell lysates (HeLa & U87MG) and in mouse tissue homogenates (liver, kidney, and embryo), as well as exogenously expressed myc-Ambra1 in lysates from transfected HEK293 cells. (Courtesy of Dr. Sabrina Di Bartolomeo, Cecconi Lab at the Dulbecco Telethon Institute, Italy).
      Western Blotting Analysis: Target specificity of a representative lot was confirmed by Western blotting analysis of Ambra1-knock mouse embryos and HeLa cells treated with Ambra1 siRNA. (Courtesy of Dr. Sabrina Di Bartolomeo, Cecconi Lab at the Dulbecco Telethon Institute, Italy).
      Western Blotting Analysis: A representative lot detected decreased Ambra1 expression in tissue homogenates (kidney, liver, and lung) from Ambra1+/- mice when compared with tissue samples from wild-type mice. (Cianfanelli, V., et al. (2015). Nat. Cell Biol. 17(1):20-30).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the C-terminus of mouse Ambra1.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the C-terminus of Ambra1. This antibody should recognize isoforms 1 through 5 based upon immunogen design.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with Human and Mouse. Predicted to react with Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • AMBRA1
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~140 kDa observed. Uniprot describes 7 isoforms at 143 kDa (isoform 1), 133 kDa (isoform 2), 87 kDa (isoform 3), 84 kDa (isoform 4), 100 kDa (isoform 5), 136 kDa (isoform 6), and 130kDa (Isoform 7). Uncharacterized band(s) may be observed in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF7 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Ambra1 in 10 µg of MCF7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      ABC131 04053252914003

      Documentation

      Anti-Ambra1 Antibody (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      Anti-Ambra1 Antibody 試験成績書（CoA）

      タイトルロット番号
      Anti-Ambra1 - 2943666 2943666
      Anti-Ambra1 - 3211897 3211897
      Anti-Ambra1 - 3379941 3379941
      Anti-Ambra1 - 3430307 3430307
      Anti-Ambra1 - 3503870 3503870
      Anti-Ambra1 - 3675891 3675891
      Anti-Ambra1 - 3739717 3739717
      Anti-Ambra1 - 3826019 3826019
      Anti-Ambra1 - 4042130 4042130
      Anti-Ambra1 - 4126635 4126635
      Show more