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MABF2325-25UL Anti-Adeno-associated virus 9 Antibody, clone HL2370

Anti-Adeno-associated virus 9, clone HL2370, Cat. No. MABF2325, is a mouse monoclonal antibody that detects Adeno-associated viruses serotype 9 capsid protein. and has been tested for use in Dot Blot, ELISA, and Neutralizing Applications.

MABF2325-25UL
25 µL  
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      概要

      Replacement Information
      Description
      Catalogue NumberMABF2325-25UL
      DescriptionAnti-Adeno-associated virus 9 Antibody, clone HL2370
      Alternate Names
      • Adeno-associated virus serotype 9
      • AAV9
      Background InformationAdeno-associated virus (AAV) is a small (25-nm), non-enveloped virus that packages a linear single-stranded DNA genome. It belongs to the family Parvoviridae and productive infection by AAV occurs only in the presence of a helper virus, which could either be adenovirus or herpesvirus. After successful infection, two stages of AAV life cycle have been described - a lytic stage and a lysogenic stage. In the presence of helper virus, the lytic stage ensues and during this period, AAV undergoes productive infection with genome replication, viral gene expression, and virion production. The lysogenic stage is established in host cells in the absence of a helper virus and AAV integrates into the host genome at specific site, AAVS1 on human chromosome19. This site is favored due to the presence of a Rep binding element. AAV genome consists of two open reading frames, Rep and Cap, flanked by two 145 base inverted terminal repeats (ITR). The ITR base pairs allow for synthesis of the complementary DNA strand. Rep and Cap are translated to produce multiple distinct proteins. The Rep gene encodes Rep78, Rep68, Rep52, Rep40 and Cap gene produces VP1, VP2, VP3 capsid proteins. The Rep78 and Rep68 proteins participate in DNA replication process via their interaction with Rep-binding element (RBE) and terminal resolution site sequences located within the ITR. The Rep52 and Rep40 proteins are involved in the generation and accumulation of single-stranded viral genomes from double-stranded replicative intermediates. Thus far eleven serotypes of AAV have been identified, which differ in their tropism or the type of cells they infect. AAVs have been used as gene delivery vectors and recombinant AAV9 has been shown to cross blood-brain barrier in neonatal and adult mice and infect astrocytes through its interaction with astrocytic perivascular endfeet in direct contact with vascular endothelial cells. Clone HL2370, generated against AAV9 capsid, specifically binds only to AAV9 and is shown to neutralize infection in cells. (Ref.: Russell, WC et al. (2009). J. Gen. Virol. 90(1); 1-20; Tseng, Y-S., et al. (2016). J. Virol. Methods. 236; 105-110).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in PBS without preservatives.
      Applications
      ApplicationAnti-Adeno-associated virus 9, clone HL2370, Cat. No. MABF2325, is a mouse monoclonal antibody that detects Adeno-associated viruses serotype 9 capsid protein. and has been tested for use in Dot Blot, ELISA, and Neutralizing Applications.
      Key Applications
      • Dot Blot
      • ELISA
      • Neutralizing
      Application NotesDot Blot Analysis: A representative lot detected Adeno-associated virus 9 in Dot Blot applicaitons (Tseng, Y.S., et. al. (2016). J Virol Methods. 236:105-110).

      Neutralizing Analysis: A representative lot neutralized Adenovirus infection in cells in Neutrailizing applicaitons (Tseng, Y.S., et. al. (2016). J Virol Methods. 236:105-110).

      ELISA Analysis: A representative lot detected Adeno-associated virus 9 in ELISA applicaitons (Tseng, Y.S., et. al. (2016). J Virol Methods. 236:105-110).
      Biological Information
      ImmunogenRecombinant Adeno-associated virus serotype 9 capsid protein.
      CloneHL2370
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone HL2370 is a mouse monoclonal antibody that specifically detects AAV9 capsid protein.
      IsotypeIgG2aκ
      Species Reactivity
      • Virus
      Species Reactivity NoteVirus (Adeno virus)
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Dot Blot with AAV8 and AAV9 virus like particles.

      Dot Blot Analysis: Various dilutions of AAV8 and AAV9 virus like particles were probed with Adenovirus 9. This antibody specifically detected AAV9, but not AAV8.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      MABF2325-25UL 04061841039136

      Documentation

      Anti-Adeno-associated virus 9 Antibody, clone HL2370 (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      Anti-Adeno-associated virus 9 Antibody, clone HL2370 試験成績書（CoA）

      タイトルロット番号
      Anti-Adenovirus 9, clone HL2370 - 4000741
      Anti-Adenovirus 9, clone HL2370 - 3277802 3277802
      Anti-Adenovirus 9, clone HL2370 - 4016289 4016289
      Anti-Adenovirus 9, clone HL2370 - 4028612 4028612
      Anti-Adenovirus 9, clone HL2370 - 4113821 4113821

      参考資料

      参考資料の概要Pub Med ID
      Generation and characterization of anti-Adeno-associated virus serotype 8 (AAV8) and anti-AAV9 monoclonal antibodies.
      Tseng, YS; Vliet, KV; Rao, L; McKenna, R; Byrne, BJ; Asokan, A; Agbandje-McKenna, M
      J Virol Methods  236  105-110  2016

      概要を表示する       		27424005 27424005