数量	カタログ番号	注文内容	Qty/Pk	価格
			96-Well Plate

数量	カタログ番号	注文内容	Qty/Pk	価格
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

194434 Sigma-AldrichABTS™ Chromophore

ABTS™ Chromophore: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

(M)SDS
試験成績書（CoA）
参考資料
Data Sheet

同義語: 2,2ʹ-Azino-bis(3-ethylbenzthiazoline-6-sulfonic Acid)

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概要

Replacement Information

価格＆在庫状況

カタログ番号	在庫状況	包装	Qty/Pk	価格	数量
194434-100MLCN	在庫状況検索中… — 現在国内在庫なし現在国内在庫有り販売中止在庫僅少現在国内在庫あり在庫を照会するにはログインしてください現在国内在庫有り現在国内在庫なし現在国内在庫有り Remaining : Will advise Remaining : Will advise 注文対象外お問合せください Contact Customer Service お問合せくださいお問合せください	ｶﾞﾗｽﾋﾞﾝ	100 ml	価格を検索中… 価格が見つかりません Minimum Quantity needs to be mulitiple of Maximum Quantity is 弊社照会詳細を表示　値引 () — 弊社照会納入価格を閲覧するにはログインしてください	在庫状況を照会 —	お気に入り/ショッピングリストに追加お気に入り/ショッピングリストに追加お気に入りに追加されました

Description
Overview	Peroxidase substrate that is soluble, safe, convenient and has good overall performance in ELISA. ABTS™ Chromophore develops intense blue-green color that can be measured at 405 nm to 410 nm. May be used for both kinetic and endpoint reactions.
Catalogue Number	194434
Brand Family	Calbiochem®
Synonyms	2,2ʹ-Azino-bis(3-ethylbenzthiazoline-6-sulfonic Acid)

References
References	Rodgriquez-Lopez, J.N., et al. 2000. Biochemistry 39, 13201.

Product Information
CAS number	28752-68-3
Declaration	ABTS is a trademark of Boehringer Mannheim Corp.
Form	Clear to very pale yellow solution
Formulation	Contains optimal concentrations of ABTS and H₂O₂ in a citrate buffer (pH 4.0) with proprietary stabilizers.
Hill Formula	C₁₈H₁₈N₄O₆S₄
Chemical formula	C₁₈H₁₈N₄O₆S₄
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Enzyme-Linked Immunosorbent Assay

Biological Information

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information
R Phrase	R: 36/37/38 Irritating to eyes, respiratory system and skin.
S Phrase	S: 26-36 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing.

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Irritant
Storage	+2°C to +8°C
Do not freeze	Ok to freeze
Special Instructions	ABTS™ solution should be stored at 4°C, but must be warmed to room temperature before use. The solution should be protected from exposure to direct sunlight, and should be discarded if it becomes bright yellow or turbid.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
カタログ番号	GTIN
194434-100MLCN	04055977221374

Documentation

ABTS™ Chromophore (M)SDS

タイトル
英語版製品安全データシート（(M)SDS）

ABTS™ Chromophore 試験成績書（CoA）

タイトル	ロット番号
194434	ロット番号を入力

参考資料

参考資料の概要
Rodgriquez-Lopez, J.N., et al. 2000. Biochemistry 39, 13201.

データシート

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	03-September-2020 JSW
Synonyms	2,2ʹ-Azino-bis(3-ethylbenzthiazoline-6-sulfonic Acid)
Description	Single component peroxidase substrate which is soluble, safe, convenient and has good overall performance for ELISA. In the presence of horseradish peroxidase (HRP) and hydrogen peroxide, ABTS is oxidized to an intense blue-green, water-soluble product. This product is a radical cation having an absorption maximum at 416 nm. Typically, product formation is measured spectrophotometrically at 405 nm, allowing a quantitative analysis of HRP-based systems. The color formation as a function of time may be monitored, or the reaction may be stopped by the addition of acid; since the acid does not alter the absorbance at 405 nm, one may perform both kinetic and endpoint measurements at the same wavelength.
Form	Clear to very pale yellow solution
Formulation	Contains optimal concentrations of ABTS and H₂O₂ in a citrate buffer (pH 4.0) with proprietary stabilizers.
Recommended reaction conditions	Suggested procedure for use of ABTS in HRP-based ELISAs: 1. Complete all required incubations with antibodies and HRP-labeled probes. 2. Wash plate wells at least 4 times with phosphate-buffered saline (PBS) or Tris-buffered saline (TBS), containing 0.1% Tween^®-20 detergent. 3. After the final wash, shake and blot all residual buffer from the wells. 4. Add 100 µl of ABTS Solution to each well and incubate at room temperature for up to 30 min. (If a kinetic study is desired, readings at 405 nm may be taken at intermediate intervals.) Note: Optimal incubation times may vary depending on the amount of HRP present. If color develops too quickly, zero order kinetics will not prevail. Dilution of the probe, antibody, or HRP-labeled reagent may be required. 5. Add 100 µl of stop solution, mix well, and read absorbance at 405 nm. [A 0.625 M oxalic acid solution is recommended as a stopping solution, as the colored product is most stable with this reagent (at least 1 h). Other acids may be employed (0.625 M oxalic acid > 0.5 M H₂SO₄ > 0.5 M HCl). 1% SDS may also be used as a stopping solution, provided that it is pure and clear (impure or turbid SDS solutions may interfere with absorbance readings).] Note: Variations in time, reagent volumes, and temperature may require further standardization by the user.
CAS number	28752-68-3
Chemical formula	C₁₈H₁₈N₄O₆S₄
Preservative	≤0.1% sodium azide
Storage	+2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	ABTS™ solution should be stored at 4°C, but must be warmed to room temperature before use. The solution should be protected from exposure to direct sunlight, and should be discarded if it becomes bright yellow or turbid.
Toxicity	Irritant
References	Rodgriquez-Lopez, J.N., et al. 2000. Biochemistry 39, 13201.

カテゴリー

Life Science Research > Protein Detection and Quantification > Immunodetection Support Products > Substrates for AP & HRP

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