Swinging Bucket Rotor			35° Fixed Angle Rotor
Amicon Ultra Device	PCR Product (base pairs)	PCR Primer (bases)	PCR Recovery (%)	PCR Primer Removal (%)	Final Volume (μL)	PCR Recovery (%)	PCR Primer Removal (%)	Final Volume (μL)
30K	137	10	92	100	37	93	100	27
		20	94	93	39	94	95	31
		48	95	71	36	93	78	31
100K	137	10	60	100	33	56	100	16
		20	50	100	33	41	100	17
		48	50	92	35	39	91	16
Spin conditions: Swinging bucket rotor, 4,000 x g for 40 min; fixed angle rotor, 7,500 x g for 15 min; room temperature, 100 μL PCR diluted to 2,000 μL starting volume, n=3.