Typical Retention of Protein Markers
Marker (Concentration) Molecular Weight Device NMWL % Retention Swinging Bucket Spin Time (min) % Retention Fixed Angle Spin Time (min)
Cytochrome c (0.25 mg/mL) 12,400 3K > 99 60 > 99 60
Cytochrome c (0.25 mg/mL) 12,400 10K > 98 40 > 99 20
BSA (1 mg/mL) 67,000 30K > 99 20 > 99 15
BSA (1 mg/mL) 67,000 50K > 96 15 > 97 10
IgG (1 mg/mL) 156,000 100K > 98 30 > 98 20
Spin Conditions: Swinging bucket rotor, 4,000 x g, or 35º fixed angle rotor, 7,500 x g, 2 mL starting volume, room temperature, n=4.