Typical Recovery of Nucleotides from Amicon-0.5 30k Devices

PCR Product (base pairs)	PCR Primer (bases)	PCR Recovery (%)	PCR Primer Removal (%)	TE Washes (number)
137	10	≥95	≥90	0
	20	≥90	≥85	1
	48	≥90	≥75	2
301	10	≥90	≥90	0
	20	≥85	≥90	1
	48	≥90	≥80	2
648	10	≥95	≥90	0
	20	≥90	≥90	1
	48	≥95	≥90	2
1159	10	≥90	≥90	0
	20	≥90	≥95	1
	48	≥95	≥95	2
Spin Conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume, 20-30 µL final volume, 10 minute spin, n=12