Typical Retention of Protein Markers
Device NMWL Marker/Concentration Molecular Weight % Retention Spin Time (min)
3K α-Chymotrypsinogen (1 mg/mL) 25,000 > 95 30
Cytochrome c (0.25 mg/mL) 12,400 > 95 30
Vitamin B-12 (0.2 mg/mL) 1,350 < 42 30
10K α-Chymotrypsinogen (1 mg/mL) 25,000 > 95 15
Cytochrome c (0.25 mg/mL) 12,400 > 95 15
Vitamin B-12 (0.2 mg/mL) 1,350 < 23 15
30K BSA (1 mg/mL) 67,000 > 95 10
Ovalbumin (1 mg/mL) 45,000 > 95 10
Cytrochorme c (0.25 mg/mL) 12,400 < 35 10
50K BSA (1 mg/mL) 67,000 > 95 10
Ovalbumin (1 mg/mL) 45,000 ~40 10
Cytrochorme c (0.25 mg/mL) 12,400 < 20 10
100K Thyroglobulin (0.5 mg/mL) 677,000 > 95 10
IgG (1 mg/mL) 156,000 > 95 10
Ovalbumin (1 mg/mL) 45,000 < 30 10
Spin Conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume, n=12.