| Method | ISO 16654 | FDA BAM Chapter 4A | FSIS MLG 5.05 |
|---|---|---|---|
| Pre-enrichment | mTSB+N, 41.5 ± 1°C; 6 and 18 -24 h |
mBPWp, 37 ± 1°C; 5 h |
mBPWp, 37 ± 1°C; 5 h |
| Selective enrichment | Immunomagnetic Concentration |
Add ACV supplement, 42 ± 1°C; 18-24 h optional immunomagnetic separation |
Immuno- magnetic separation |
| Isolation | CT-SMAC agar, 37°C; 18-24 h |
CT-SMAC Agar + chromogenic agar (Rainbow O157 Agar or R&F E.coli O157 Agar), 37 ± 1°C; 18-24 h |
Rainbow O157 Agar, 35 ± 2°C; 24-26h |
大腸菌O157/EHEC/STEC/VTEC検査
大腸菌O157/EHEC/STEC/VTEC検出用の
|
資料請求 |
病原性大腸菌のうち、ベロ毒素を産生する菌株（STECまたはVTEC）、特に病原性の高い血清型O157:H7菌株の腸管出血性大腸菌（EHEC）グループが近年重要性を増しています。主な感染源は、汚染された生または加熱が不十分な肉、乳製品といった動物由来の食品です。EHECは、特に免疫不全のある人や幼児、高齢者で生命を脅かす疾患を引き起こすことがあります。近年、大腸菌O157による食品汚染の発生が大幅に増加しています。
Merckは、ベロ毒素産生性大腸菌株を増菌、検出および分離するための製品を幅広く取り揃えています。具体的には、以下の通りです。
- 顆粒状培地：ISO 11133に規定された業界最高レベルの性能基準を満たした、独自の便利な顆粒状培地
- シングルパス® ラテラルフロー検査：増菌後わずか20分で検出できる、非常に便利な「妊娠検査キット」と同原理の革新的な免疫検査法
E. coli are Gram-negative, non-sporulating, facultative anaerobic bacilli. They can grow on a wide range of substrates and at temperatures ranging from below 15°C up to 45°C or even higher, as seen with certain laboratory strains (optimum temperature 37°C). Some strains are motile and possess flagella.
Amongst the pathogenic E. coli, Verotoxin-forming strains (STEC or VTEC) have gained importance in recent years. The group of enterohaemorrhagic E. coli (EHEC) with its highly pathogenic serovar O157:H7 strain is particularly interesting in this respect. The main sources of infection are contaminated, raw or insufficiently heated foods of animal origin, such as meat and dairy products. The reservoir for EHEC is the gut of ruminants. The microorganisms can enter food during the processing of meat and dairy products if hygienic conditions are inadequate. The drastic increase in the incidence of food contamination caused by E. coli O157 demands reliable and rapid methods of detection.
EHEC Infection
EHEC is capable of inducing life threatening illnesses, particularly in people with immune deficiency, young children and the elderly. Although the most common cause of EHEC is E. coli O157, other serotypes, such as O26, O45, O103, O111, O121, and O145, are also relatively frequent causes of infection.
Typical symptoms are severe abdominal pain and diarrhea, which is initially watery but often becomes bloody. These are occasionally accompanied by vomiting. The illness is usually self-limited and lasts for an average of 8 days. In up to 10% of all cases, kidney complications (so-called hemolytic-uremic syndrome or HUS) occur, which can lead to temporary or even permanent kidney damage. Neurological symptoms may also occur.
