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70767 pET Expression System 23 - Novagen

pET vectors plus host strains with induction control

pET Expression System 23 - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70767
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      Description
      OverviewpET Expression Systems and pET
      pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

      Components: pET Expression Systems
      Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
      • 10 µg pET vector DNA (for each indicated plasmid)
      • 0.2 ml BL21 Glycerol Stock
      • 0.2 ml BL21(DE3) Glycerol Stock
      • 0.2 ml BL21(DE3)pLysS Glycerol Stock
      • 0.2 ml Induction Control Glycerol Stock

      Components: pET Expression Systems plus Competent Cells
      pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
      • 0.2 ml NovaBlue Competent Cells
      • 0.2 ml BL21(DE3) Competent Cells
      • 0.2 ml BL21(DE3)pLysS Competent Cells
      • 2 × 0.2 ml SOC Medium
      • 10 µl Test Plasmid

      These components are sufficient for up to 10 transformations in each host.

      Purification and Detection Reagents
      Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

      pET Expression System 23
      The pET Expression System 23 contains 10 µg each of the four versions of pET-23 (pET-23a–d(+)). pET-23(+) is a transcription vector designed for expression from bacterial translation signals carried within a cloned insert. It therefore lacks the ribosome binding site and ATG start codon present on the pET translation vectors. A C-terminal His•Tag® sequence is available. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB051VM.




      Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number70767
      Brand Family Novagen®
      References
      Product Information
      •10 µg eachpET-23a-d(+) vector DNA
      •0.2 mlHost bacterial strains BL21, BL21(DE3), and BL21(DE3)pLysS, glycerol stocks
      •0.2 mlInduction control clone, glycerol stock
      Fusion tagT7•Tag, His•Tag
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      70767-3CN 04055977273199

      Documentation

      pET Expression System 23 - Novagen Certificates of Analysis

      TitleLot Number
      70767

      Citations

      Title
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    • Cishan Li, Michael E. Salvucci and Archie R. Portis, Jr.. (2005) Two residues of rubisco activase involved in recognition of the rubisco substrate. Journal of Biological Chemistry 280, 24864-24869.
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    • Eugenia Mata-Greenwood, et al. (2005) Cyclic stretch increases VEGF expression in pulmonary arterial smooth muscle cells via TGF-{beta}1 and reactive oxygen species: a requirement for NAD(P)H oxidase. American Journal of Physiology: Lung 289, L288-L289.
    • Prachee Prakash, et al. (2005) Purified recombinant hypothetical protein coded by open reading frame Rv1885c of Mycobacterium tuberculosis exhibits a monofunctional AroQ class of periplasmic chorismate mutase activity. Journal of Biological Chemistry 280, 19641-19648.
    • Sadayappan Sakthivel, et al. (2005) In vivo and in vitro analysis of cardiac troponin I phosphorylation. Journal of Biological Chemistry 280, 703-714.
    • P. Szweda, R. Kotlowski and J. Kur. (2005) New effective sources of the Staphylococcus simulans lysostaphin. Journal of Biotechnology 117, 203-213.
    • Olga Vitavska, Hans Merzendorfer and Helmut Wieczorek. (2005) The V-ATPase Subunit C Binds to Polymeric F-actin as Well as to Monomeric G-actin and Induces Cross-linking of Actin Filaments. Journal of Biological Chemistry 280, 1070-1076.
    • Naohide Watanabe and Eric Lam. (2005) Two Arabidopsis metacaspases AtMCP1b and AtMCP2b are arginine/lysine-specific cysteine proteases and activate apoptosis-like cell death in yeast. Journal of Biological Chemistry 280, 14691-14699.
    • Bonney Wilkinson, Ruoyu Xiao and Hiram F. Gilbert. (2005) A structural disulfide of yeast protein disulfide isomerase destabilizes the active site disulfide of the N-terminal thioredoxin domain. Journal of Biological Chemistry 280, 11483-11487.
    • Liangwen Xiong, et al. (2005) Sites on calmodulin that interact with the C-terminal tail of Cav1.2 channel. Journal of Biological Chemistry 280, 7070-7079.
    • Hisashi Yagi, et al. (2005) Amyloid fibril formation of α-synuclein is accelerated by preformed amyloid seeds of other proteins: Implications for the mechanism of transmissible conformational diseases. Journal of Biological Chemistry 280, 38609-38616.
    • Roger B. Dodd, et al. (2004) Solution structure of the Kaposi's sarcoma-associated herpesvirus K3 N-terminal domain reveals a Novel E2-binding C4HC3-type RING. Journal of Biological Chemistry 279, 53840-53847.
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    • Dan Gu, et al. (2004) Identification and characterization of the DNA-binding domain of the multifunctional PutA flavoenzyme. Journal of Biological Chemistry 279, 31171-31176.
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    • Assen Marintchev, Michael R. Gryk and Gregory P. Mullen. (2003) Site-directed mutagenesis analysis of the structural interaction of the single-strand-break repair protein, X-ray cross-complementing group1, with DNA polymerase β. Nucleic Acids Research 31, 580-588.
    • Cedric Orelle, et al. (2003) The conserved glutamate residue adjacent to the Walker-B motif is the catalytic base for ATP hydrolysis in the ATP-binding cassette transporter BmrA. Journal of Biological Chemistry 278, 47002-47008.
    • Yumi Maeda, et al. (2002) Novel 33-kilodalton lipoprotein from Mycobacterium leprae. Infection and Immunity 70, 4106-4111.
    • Diane C. Bassham, et al. (2000) AtVPS45 complex formation at the trans-golgi network. Molecular Biology of the Cell 11, 2251-2265.
    • Quinn L. Deveraux, et al. (1998) IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases. European Molecular Biology Organization Journal 17, 2215-2223.
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    • K.K. Zimmerman, et al. (1998) High-level expression of rat farnesyl:protein transferase in Escherichia coli as a translationally coupled heterodimer. Protein Expression and Purification 14, (395- 402).
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      		•

      User Protocols

      Title
      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB055 pET System Manual

      Vector Map

      Title
      TB051VM pET-23a-d(+) Vector Map

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