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70003 pBAC™-1 DNA - Novagen

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70003
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70003-3CN
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      Plastic ampoule 10 μg
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      Description
      OverviewpBAC™-1 is a baculovirus transfer plasmid designed for simplified cloning and expression of target genes in insect cells. The plasmid is compatible with BacVector™-1000, -2000 or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the polh promoter. Inserts in pBAC-1 must contain their own translation initiation codon. The vector encodes an optional C-terminal His•Tag® fusion sequence that may be utilized if the insert allows read-through in the proper reading frame. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Single stranded sequencing of phage-derived DNA can be performed using the 1629DWN primer.





      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      Catalogue Number70003
      Brand Family Novagen®
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      70003-3CN 04055977273830

      Documentation

      pBAC™-1 DNA - Novagen SDS

      Title

      Safety Data Sheet (SDS) 

      pBAC™-1 DNA - Novagen Certificates of Analysis

      TitleLot Number
      70003

      Brochure

      Title
      Insect Cell Expression

      Citations

      Title
    • Zhongmin Ma, et al. (1999) Human pancreatic islets express RNA species encoding two distinct catalytically active isoforms of group VI phospholipase A2 (iPLA2) that arise from an exon-skipping mechanism of alternative splicing of the transcript from the iPLA2 gene on the chromosome. Journal of Biological Chemistry 274, 9607-9616.
    • Antonio Alcami, et al. (1998) Blockade of chemokine activity by a soluble chemokine binding protein from vaccinia virus. Journal of Immunology 160, 624-633.
    • Robert Bals, et al. (1998) Human defensin 2 Is a salt-sensitive peptide antibiotic expressed in human lung. Journal of Clinical Investigation 102, 874-880.
    • Vector Map

      Title
      TB127 pBAC-1 Vector Map

      Vector Sequence

      Title
      pBAC-1 Sequence

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Insect Expression > Insect Expression Vectors & Kits