Immobilon^® Block - PO (Phosphoprotein Blocker)

The possible causes and potential remedies for challenges encountered as a result of blocking, washing, antibody incubation, and detection/exposure of Western blots

We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise - except that it will spark creativity in the lab.

我々はそれを理解している。ウエスタンはしみ抜きで苦労することがある。そこで、あなたの醜いブロットを私たちと共有し、サプライズを受け取ってください！それが何であるかは言えませんが-それは驚きを台無しにしてしまうからです-ラボの創造性を掻き立てるということを除いては。

In Western blotting, blocking of unbound membrane sites is necessary to prevent non-specific binding of the antibodies which otherwise would lead to a high background on the blot. The traditional milk blocker can work well; however, inadvertent variations in its composition can lead to irreproducible results. Such variations include the milk’s concentration, fat content, solubility, detergent quality, and numerous other factors. Bløk reagents are a family of protein-free, noisecancelling reagents that reduce background for consistent, quality results. They are available in three room temperature-stable, ready-to-use formulations for Chemiluminescence and chromogenic detection, Fluorescence detection, and Phosphoprotein immunodetection. The protein-free nature of Bløk reagents allows for membranes to be stained with chromogenic reagents, such as Ponceau S or Coomassie™ blue, after blocking or immunodetection. Bløk reagents have been tested and validated for Western blot, dot blot, and ELISA applications.