NS170
Multi Species Phosphorylated Neurofilament ELISA Kit
measures and quantifies pNF-H levels in serum or plasma
Synonym(s):
200 kDa neurofilament protein, Phosphorylated Neurofilament heavy polypeptide, pNF-H
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
Product Name
Phosphorylated Neurofilament (pNF-H) Sandwich ELISA Kit, The Phosphorylated Neurofilament H (pNF-H) kit is a sandwich enzyme immunoassay (EIA), which measures pNF-H.
species reactivity
pig, feline, rat, human, bovine, mouse
manufacturer/tradename
Chemicon®
assay range
sensitivity: 0.0293-15 ng/mL
(Range of Detection)
sensitivity: 0.0585 ng/mL
technique(s)
ELISA: suitable
NCBI accession no.
UniProt accession no.
application(s)
research use
detection method
colorimetric
shipped in
dry ice
Quality Level
Application
Research Category
Neuroscience
Neuroscience
The Phosphorylated Neurofilament H (pNF-H) kit is a sandwich enzyme immunoassay (EIA), which measures pNF-H.
Used to detect/quantify: Phosphorylated Neurofilament (pNF-H)
Disclaimer
For research use only. Not for use in diagnostic procedures.
Packaging
96 wells
Preparation Note
Prior to use maintain the kit components at 2°-8°C and -20°C, as indicated, until expiration date.
1. Wash Buffer - After dilution maintain the Wash Solution at 2°-8°C for up to one month or until expiration date.
2. Tween® 20 - Store at room temperature upon receipt of kit.
3. Elisa Assay Buffer - Aliquot as required to avoid repeated freeze-thaw cycles. After thawing, maintain the Elisa Assay Buffer at 2°-8°C for up to one month, or until expiration date.
4. Immunoplate Pre-coated with Chicken anti- pNF-H Polyclonal Antibody - Unused strip wells may be returned to the foil pouch and stored at 2°-8°C for up to one month, or until expiration date.
1. Wash Buffer - After dilution maintain the Wash Solution at 2°-8°C for up to one month or until expiration date.
2. Tween® 20 - Store at room temperature upon receipt of kit.
3. Elisa Assay Buffer - Aliquot as required to avoid repeated freeze-thaw cycles. After thawing, maintain the Elisa Assay Buffer at 2°-8°C for up to one month, or until expiration date.
4. Immunoplate Pre-coated with Chicken anti- pNF-H Polyclonal Antibody - Unused strip wells may be returned to the foil pouch and stored at 2°-8°C for up to one month, or until expiration date.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
Other Notes
Immunoplate Pre-coated with Chicken anti-pNF-H Polyclonal Antibody: (Part No. 2006263). One 96-well immunoplate pre-coated with chicken anti- pNF-H polyclonal antibody sealed in a foil pouch.
Rabbit anti- pNF-H Polyclonal Antibody: (Part No. 2006919). One vial (100 μL) of rabbit anti- pNF-H polyclonal antibody.
Goat anti-Rabbit, Alkaline Phosphatase Conjugated Antibody: (Part No. 2006917). One vial (10 μL) of goat anti-rabbit alkaline phosphatase conjugate.
Bovine pNF-H Standard: (Part No. 2006918). One vial (5.0 ng) of bovine pNF-H standard.
20X TBS: (Part No. 2004913). One bottle (50 mL) of 20X TBS.
20% Tween® 20: (Part No. 2004915). One vial (3 mL) of 20% Tween-20.
Elisa Assay Diluent: (Part No. 2006921) One bottle (30 mL) of Elisa assay diluent containg preservative, provided in a ready to use format.
pNPP Buffer: (Part No. 2006261). One bottle (12 mL) of pNPP buffer for use with 50X p-NPP, provided in a ready to use format.
50X pNPP: (Part No. 2006262). One vial (250 μL) of 50X pNPP (p-nitrophenyl phosphatase) alkaline phosphatase substrate.
Plate Covers - Two Plate Covers.
Rabbit anti- pNF-H Polyclonal Antibody: (Part No. 2006919). One vial (100 μL) of rabbit anti- pNF-H polyclonal antibody.
Goat anti-Rabbit, Alkaline Phosphatase Conjugated Antibody: (Part No. 2006917). One vial (10 μL) of goat anti-rabbit alkaline phosphatase conjugate.
Bovine pNF-H Standard: (Part No. 2006918). One vial (5.0 ng) of bovine pNF-H standard.
20X TBS: (Part No. 2004913). One bottle (50 mL) of 20X TBS.
20% Tween® 20: (Part No. 2004915). One vial (3 mL) of 20% Tween-20.
Elisa Assay Diluent: (Part No. 2006921) One bottle (30 mL) of Elisa assay diluent containg preservative, provided in a ready to use format.
pNPP Buffer: (Part No. 2006261). One bottle (12 mL) of pNPP buffer for use with 50X p-NPP, provided in a ready to use format.
50X pNPP: (Part No. 2006262). One vial (250 μL) of 50X pNPP (p-nitrophenyl phosphatase) alkaline phosphatase substrate.
Plate Covers - Two Plate Covers.
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral
target_organs
Kidney,Liver,Blood
Storage Class
10 - Combustible liquids
wgk
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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A systematic review and meta-analysis of CSF neurofilament protein levels as biomarkers in dementia.
A Petzold et al.
Neuro-degenerative diseases, 4(2-3), 185-194 (2007-06-29)
Loss of cortical neurons is a key pathological feature in neurodegenerative dementias. Cerebrospinal fluid (CSF) neurofilaments (Nf) are a biomarker for neuronal death and axonal loss. To perform a meta-analysis to investigate the value of CSF Nf levels for the
P N Hoffman et al.
The Journal of cell biology, 66(2), 351-366 (1975-08-01)
This study of the slow component of axonal transport was aimed at two problems: the specific identification of polypeptides transported into the axon from the cell body, and the identification of structural polypeptides of the axoplasm. The axonal transport paradigm
Gerry Shaw et al.
Biochemical and biophysical research communications, 336(4), 1268-1277 (2005-09-24)
Several lines of reasoning suggest that the phosphorylated axonal form of the neurofilament subunit NF-H is likely to be released from damaged and diseased neurons in significant amounts. Detection of this protein in serum or CSF might therefore provide information
Kevin J Anderson et al.
Journal of neurotrauma, 25(9), 1079-1085 (2008-08-30)
The detection of neuron-specific proteins in blood might allow quantification of the degree of neuropathology in experimental and clinical contexts. We have been studying a novel blood biomarker of axonal injury, the heavily phosphorylated axonal form of the high molecular
A Petzold et al.
Journal of neurology, neurosurgery, and psychiatry, 75(8), 1178-1180 (2004-07-20)
To investigate whether plasma biomarkers for axonal injury and inflammation are related to loss and recovery of visual function in acute optic neuritis (ON). Eighteen patients with ON and 14 controls were investigated in a longitudinal, prospective study. Plasma phosphorylated
Global Trade Item Number
| SKU | GTIN |
|---|---|
| NS170 | 04053252010699 |
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