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Merck

1.11972

Tryptose sulfite cycloserine agar (base)

GranuCult® prime, according to ISO 15213, ISO 14189, AOAC®, APHA, FDA-BAM, granular, for Clostridium spp., pkg of 500 g

Synonym(s):

TSC agar

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About This Item

NACRES:
NA.74
UNSPSC Code:
41106213
Manufacturer/tradename:
GranuCult® prime
Agency:
AOAC®
APHA
FDA-BAM
ISO 14189
according to ISO 15213
Ph:
7.4-7.8 (25 °C in H2O)
Form:
granular
Suitability:
Clostridium spp.
Application(s):
food and beverages
microbiology

Key Documents

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Product Name

Tryptose sulfite cycloserine agar (base), GranuCult® prime, according to ISO 15213, ISO 14189, AOAC®, APHA, FDA-BAM, granular, for Clostridium spp., pkg of 500 g

agency

AOAC®
APHA
FDA-BAM
ISO 14189
according to ISO 15213

Quality Level

200

storage temp.

15-25°C

suitability

Clostridium spp.

sterility

non-sterile

form

granular

packaging

pkg of 500 g

manufacturer/tradename

GranuCult® prime

technique(s)

microbiological culture: suitable

pH

7.4-7.8 (25 °C in H2O)

solubility

42 g/L

bulk density

660 kg/m3

application(s)

food and beverages
microbiology

compatibility

for use with Clostridium perfringens selective supplement (1.00888)

Related Categories

Application

GranuCult® Tryptose sulfite cycloserine agar (base) is intended for the isolation and differentiation of Clostridium perfringens from food and animal feed, water and other materials. TSC agar with MUP can be prepared using Clostridium perfringens Supplement (1.00888) to detect acid phosphatase by its fluorescence under UV light.

Disclaimer

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

General description

GranuCult® Tryptose sulfite cycloserine agar (base) is intended for the isolation and differentiation of Clostridium perfringens. The superior nutrient base provides optimal conditions for the development of Clostridia. Colonies producing hydrogen sulfide are characterized by blackening due to the reaction with sulfite and iron salt. Cycloserine inhibits the accompanying bacterial flora and may cause the colonies that develop to remain smaller. It also reduces diffusion and thus the disturbing blackening around the Clostridium perfringens colonies. TSC agar with MUP contains a fluorogenic substrate for the alkaline and acid phosphatase in addition to cycloserine 4-Methylumbelliferylphosphate (MUP). The acid phosphatase is a high specific indicator for Clostridium perfringens. It splits the fluorogenic substrate MUP, forming 4-methylumbelliferone, which can be identified by its fluorescence in long wave UV light. Thus, a strong suggestion for the presence of Clostridium perfringens can be obtained.

Legal Information

AOAC is a registered trademark of AOAC International
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
1.11972.050004022536137773

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