507858
PANSORBIN® Cells
Synonym(s):
Staphylococcus aureus Cells
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About This Item
NACRES:
NA.42
UNSPSC Code:
41116133
Manufacturer/tradename:
Calbiochem®
form
suspension (Homogeneous)
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
impurities
≤2500 cells/mL Viable cells
color
milky
capacity
≥2.0 mg(human IgG/100 mg cells)
shipped in
ambient
storage temp.
2-8°C
Quality Level
Disclaimer
Toxicity: Standard Handling (A)
General description
PANSORBIN Cells are heat-killed, formalin-fixed Staphylococcus aureus cells that have a coat of protein A and have been pickled by the method of Kessler. Useful for mitogenic stimulation of B lyphocytes and for immunoprecipitation.
Heat-killed, formalin-fixed Staphylococcus aureus cells (Cowan I strain) that bear a high cell-surface density of protein A and have been pickled by the method of Kessler. Useful as a solid-phase IgG-binding reagent due to the high affinity interaction of protein A with the Fc domain of IgG. PANSORBIN cells work best when the antibody is human (IgG1, IgG2, IgG4), rabbit IgG (all isotypes), or mouse (IgG2a, IgG2b, IgG3). Most common applications include immunoprecipitation and mitogenic stimulation of B lymphocytes.
Other Notes
Kierszenbaum, F., et al. 1991. Immunology74, 317.
Meikle, P.J., et al. 1991. J. Biol. Chem.266, 22569.
Ezaki, O., et al. 1989. Biochem. Biophys. Res. Commun. 159, 1368.
Murakami, H., et al. 1988. Biochem. J.256, 917.
Kessler, S.W. 1975. J. Immunol. 115, 1617.
Meikle, P.J., et al. 1991. J. Biol. Chem.266, 22569.
Ezaki, O., et al. 1989. Biochem. Biophys. Res. Commun. 159, 1368.
Murakami, H., et al. 1988. Biochem. J.256, 917.
Kessler, S.W. 1975. J. Immunol. 115, 1617.
Physical form
Supplied as a 10% suspension of Staphylococcus aureus cells in PBS, 0.1% NaN₃, pH 7.2.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PANSORBIN is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
507858-50GM: + 507858-10GM: + 507858-1GM: + 507858-GM: + 507858-5GM:
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Certificates of Analysis (COA)
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Derek Walsh et al.
Genes & development, 20(4), 461-472 (2006-02-17)
Recruitment of the 40S ribosome to the 5' end of a eukaryotic mRNA requires assembly of translation initiation factors eIF4E, the cap-binding protein, together with eIF4A and eIF4G into a complex termed eIF4F. While the translational repressor 4E-BP1 regulates binding
J R Faust et al.
Proceedings of the National Academy of Sciences of the United States of America, 79(17), 5205-5209 (1982-09-01)
UT-1 cells are a clone of Chinese hamster ovary cells that were selected to grow in the presence of compactin, a competitive inhibitor of 3-hydroxy-3-methylglutaryl-coenzyme A reductase [mevalonate: NADP+ oxidoreductase (CoA-acylating), EC 1.1.1.34]. These cells have 100- to 1,000-fold more
Peter Yaciuk
Methods in molecular medicine, 131, 103-111 (2007-07-28)
Co-immunoprecipitation is a common method used to determine protein-protein interactions. This method typically exploits the highly specific interaction between a monoclonal antibody and a protein of interest. Providing that the antibody-protein interaction does not interfere with the ability of the
C S Abrams et al.
The Journal of biological chemistry, 270(1), 333-339 (1995-01-06)
The Src homology 2 (SH2) and Src homology 3 (SH3) domain are approximately 50% conserved in various Src family kinase members. Several lines of evidence suggest that in Src these domains are sequence motifs that direct substrate recognition, regulate kinase
Leah S Bernstein et al.
Methods in molecular biology (Clifton, N.J.), 237, 195-204 (2003-09-23)
Palmitoylation refers to the covalent attachment of a 16-carbon fatty acid to cysteine residues of proteins. This modification occurs on many intracellular signaling proteins including regulators of G protein signaling proteins (RGS). Palmitoylation mediates the interaction of proteins with membranes
Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 507858-50GMCN | 04055977271942 |
| 507858-1GMCN | 04055977272499 |
| 507858-10GMCN | 04055977272505 |
| 507858-5GMCN | 04055977271935 |
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