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Merck

407294

αA-Interferon, Human, Recombinant, E. coli

Synonym(s):

IFNA2A, IFNA2B, IFNA2C, rhIFN-αA

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About This Item

NACRES:
NA.77
UNSPSC Code:
12352202

Key Documents

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description

Merck USA index - 14, 4990

assay

≥95% (SDS-PAGE)

form

liquid

specific activity

≥2.8 x 108 units/mg protein
≥5 x 106 units/mL

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

technique(s)

cell based assay: suitable

solubility

soluble (Dilute only in buffers containing protein such as 0.1% BSA.)

NCBI accession no.

NM_000605.4

UniProt accession no.

P01563

shipped in

wet ice

storage temp.

−70°C

Quality Level

100

Gene Information

human ... IFNA1(3340)

Application

Interferon alpha has been used to evaluate its effect on VSV (vesicular stomatitis virus) infection in pancreatic ductal adenocarcinoma (PDA) cell lines and study the relationship between type I IFNsignaling, resistance to VSV infection, and the JAK/STAT (Janus kinase/signal transducers and activators of transcription) signaling pathways in PDA cell lines. It has also been used to study the inhibitory effect of Morbillivirus V Proteins on Interferon Signaling Pathways.

Biochem/physiol Actions

Recombinant, human αA-interferon expressed in E. coli have antiviral, antiproliferative, antineoplastic, and immunomodulating properties. Recombinant interferon αA (IFN-αA) is used as the first line of treatment for hepatitis C. It also displays a great therapeutic potential with chronic viral hepatitis, polycythemia, leukemia, and various other malignancies. It enhances natural killer cell activity, suppresses bone marrow function, and induces apoptotic cell death in non-melanoma skin cancer.

Disclaimer

Toxicity: Standard Handling (A)

General description

Research area: Immunoand cks

Interferons are cytokines secreted by mammalian cells. They are broadly divided into type I– interferon alpha (IFN-α) and interferon beta (IFN-β), type II – interferon-gamma (IFN-γ), and type III – interferon lambda (IFN-λ) based on their structure, function, and receptor specificity. Recombinant αA interferon is a hydrophobic protein belonging to the IFN-α family of proteins.

Other Notes

Fujishima, H., et al. 1998. Int. J. Cancer 76, 423.
Haria, M., and Benfield, P. 1995. Drugs 50, 873.
Familletti, P.C., et al. 1981. Methods Enzymol. 78, 387.
One unit of interferon per ml of medium will cause a 50% inhibition of VSV-induced cytopathic effect on bovine MDBK cells. Units are determined with respect to the NIH international reference standard for human αA-IFN. Dilute in aqueous buffer containing at least 0.1% BSA or HSA.

Physical form

In PBS containing 0.1% BSA.

Preparation Note

Dilute only in buffers containing protein such as 0.1% BSA.
Following initial thaw, aliquot and freeze (-70°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Expression of Soluble Active Interferon ?A in Escherichia coli Periplasm by Fusion with Thermostable Lichenase Using the Domain Insertion Approach
Tyurin AA, et al.
Biochemistry, 83(3), 259-269 (2018)
Morbillivirus V Proteins Exhibit Multiple Mechanisms to Block Type 1 and Type 2 Interferon Signalling Pathways
Senthil K, et al.
PLoS ONE, 8(2), e57063-e57063 null
Resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus: role of type I interferon signaling
Moerdyk-Schauwecker M, et al.
Virology, 436(1), 221-234 (2013)

Global Trade Item Number

SKUGTIN
407294-5MUCN04055977188202

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