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SCC468 T24T Human Bladder Carcinoma Cell Line

T24T human bladder cancer cell line is a useful model for the study of metastatic bladder cancer

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SCC468
≥1X10⁶ cells/vial   
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCC468
      DescriptionT24T Human Bladder Carcinoma Cell Line
      Background InformationBladder cancer originates from the epithelium that covers the inner surface of the bladder. The most common type is urothelial or transitional carcinoma (1). Most of the newly diagnosed cases (75%) are non-invasive, if the tumor becomes invasive it is more likely to metastasize to lymph nodes and other organs. Two common alterations have been identified in non-invasive tumors, deletion of chromosome 9 and a point mutation in FGFR3 (Fibroblast growth factor receptor 3) (2). Activating mutations in RAS gene family members are also common in bladder carcinoma and it is estimated that activation of the RAS-MAPK pathway may contribute to over 80% of the non-invasive cases (1). Loss of function of tumor suppressor genes like TP53 and RB1 are common in cases of invasive disease (1). After diagnosis the patient requires resection of the tumor and in cases of invasive disease chemotherapy is also provided.

      The original cell line T24 has been used to study bladder tumor biology but has some limitations because it lacks tumorigenicity when injected into immunocompromised mice. In contrast, T24T has increased metastatic ability when tested in vivo and expresses higher levels of HRAS mRNA (3). Increased HRAS activity leads to focal adhesion disassembly and los of CDH1 and b-catenin from intercellular junctions (3). Contact inhibition growth is reduced in T24T compared with T24 (3). Additionally, T24T does not inhibit its motile activity at higher density like T24 does.

      Source
      T24 cell line was generated from an invasive human transitional cell carcinoma of the bladder (4). T24T is a derivative of T24.

      References
      1. Nat Rev Dis Primers 2017, 3: 17022.
      2. Int J Cancer 2006, 119(5): 1212-1215.
      3. Genes Chromosomes Cancer 2000, 27(3): 252-263.
      4. Int J Cancer 1973, 11(3): 765-773.
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationT24T human bladder cancer cell line is a useful model for the study of metastatic bladder cancer
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Cancer Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEach vial contains ≥1X10⁶ viable cells.

      Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.

      Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, Chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services

      Cells are negative for mycoplasma contamination.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCC468 04065267040004