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A-004-C Poly-L-Ornithine Solution (0.01%)

Poly-L-Ornithine Solution (0.01%) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Attachment, Cell Culture, Neuroscience
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A-004-C
100 ml  
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      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      Cell Attachment, CULT, Neuroscience
      Description
      Catalogue NumberA-004-C
      DescriptionPoly-L-Ornithine Solution (0.01%)
      Alternate Names
      • Ornithine
      Background InformationPoly-L-Ornithine is a synthetic amino acid chain that is positively charged having one hydrobromide per unit of ornithine. Poly-L-Ornithine is widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. Poly-L-Ornithine is often used in combination with laminin for many types of neuronal and neural stem cell cultures.

      The molecular weight of Poly-L-Ornithine for cell culture can vary significantly, with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (greater than 300,000 Da) having more binding sites per molecule. This product uses a Poly-L-Ornithine of 78000 Da, yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.
      References
      Product Information
      Formulation
      PresentationProduct is presented in sterile water at 0.01% w/v (0.1mg/ml). Product is filtered through a 0.2 micron filter before freezing.
      Quality LevelMQ100
      Applications
      Key Applications
      • Cell Attachment
      • Cell Culture
      • Neuroscience
      Application NotesOptimal coating concentrations must be determined by end user. Typical coating concentrations range from 10ug/ml to 100ug/ml based on cell type and application.

      1. Thaw Poly-L-Ornithine solution at room temperature.
      2. Dilute Poly-L-Ornithine solution to desired concentration in sterile water.
      3. Fully coat the cell culture surface with diluted Poly-L-Ornithine solution. Use 5 mL volume for 6-cm plates and 10 mL volume for 10-cm plates and T75 flasks.
      4. Allow cell culture vessel to sit at room temperature overnight.
      5. Aspirate the Poly-L-Ornithine solution the following day and rinse vessel with sterile water followed by coating with desired ECM protein.
      Biological Information
      Concentration0.01% w/v (0.1 mg/ml)
      Media FormLiquid
      Molecular Weight78000 Da
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceSolution is clear with no particulates present. Solution is sterile and suitable for cell culture applications.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20°C for up to 4 months from date of receipt. Avoid multiple freeze thaw cycles to maintain product integrity.
      Packaging Information
      Material Size100 ml
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      A-004-C 04053252915000