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Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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524736
Sigma-AldrichPhosphodiesterase 4B2, His•Tag®, Human, Recombinant, S. frugiperda
Phosphodiesterase 4B2, His•Tag®, Human, Recombinant, S. frugiperda MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview
Full-length, recombinant, human phosphodiesterase 4B2 (accession no. L20971) fused at the N-terminus to a His•Tag® sequence and expressed in S. frugiperda using a baculovirus expression system. Plays a significant role in the regulation of inflammatory cell activation. PDE4B2 is a predominant isoform in neutrophils and monocytes. Supplied as a partially purified, catalytically active preparation.
Catalogue Number
524736
Brand Family
Calbiochem®
Synonyms
PDE4B2, cAMP-Specific Phosphodiesterase 4B2
Application Data
Phosphodiesterase 4B2 was treated with increasing concentrations of the inhibitor, rolipram, and the activity assayed. the IC50 for this inhibition is 1.77 µM.
References
References
Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol.538, 39. Smith, P.G., et al. 2005 Blood105, 308. Baroja, M.L., et al. 1999. J. Immunol.162, 2016.
Product Information
Activity
≥ 0.25 units/µl
Unit of Definition
One unit is defined as the amount of enzyme that will convert 1 pmol cAMP to AMP per min at 30°C, pH 7.5.
Phosphodiesterase 4B2, His•Tag®, Human, Recombinant, S. frugiperda Certificates of Analysis
Title
Lot Number
524736
References
Reference overview
Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol.538, 39. Smith, P.G., et al. 2005 Blood105, 308. Baroja, M.L., et al. 1999. J. Immunol.162, 2016.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
10-March-2009 JSW
Synonyms
PDE4B2, cAMP-Specific Phosphodiesterase 4B2
Application Data
Phosphodiesterase 4B2 was treated with increasing concentrations of the inhibitor, rolipram, and the activity assayed. the IC50 for this inhibition is 1.77 µM.
Description
Full-length, recombinant, human phosphodiesterase 4B2 (accession no. L20971) fused at the N-terminus to a His•Tag® sequence and expressed in S. frugiperda using a baculovirus expression system. Plays a significant role in the regulation of inflammatory cell activation. PDE4B2 is a predominant isoform in neutrophils and monocytes. Supplied as a partially purified, catalytically active preparation.
Form
Liquid
Formulation
In 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.
Activity
≥ 0.25 units/µl
Unit definition
One unit is defined as the amount of enzyme that will convert 1 pmol cAMP to AMP per min at 30°C, pH 7.5.
Storage
Avoid freeze/thaw ≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-70°C).
Toxicity
Standard Handling
References
Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol.538, 39. Smith, P.G., et al. 2005 Blood105, 308. Baroja, M.L., et al. 1999. J. Immunol.162, 2016.
