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109253 Papanicolaou's solution 1a Harris' hematoxylin solution

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109253
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      1.09253.1000
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          Description
          Catalogue Number109253
          SynonymsHematoxylin solution acc. to Harris, Colour solution acc. to Papanicolaou, Harris' Hematoxylin solution
          DescriptionPapanicolaou’s solution 1a Harris hematoxylin solution
          OverviewPapanicolaou’s solution 1a Harris hematoxylin solution - for cytology, is a ready-to-use solution used for human-medical cell diagnosis and serve the purpose of the cytological investigation of sample material of human origin, for example, gynecological and clinico-cytological specimen materials. Papanicolaou’s technique is the most used staining procedure for cytological specimen is and is intended for the the staining of exfoliative cells in cytological specimens. In the first step, the cell nuclei are stained either progressively or regressively with a hematoxylin solution, (Hematoxylin solution modified acc.to Gill II, (Cat.no. 105175), Papanicolaou’s solution 1a Harris hematoxylin solution, (Cat.no. 109253) or Papanicolaou’s solution 1b Hematoxylin solution S, (Cat.no. 109254)). Nuclei are stained blue to dark violet. In the progressive hematoxylin staining method, staining is carried out to the endpoint, after which the slide is blued in tapwater. With the regressive method the material is over-stained and the excess of staining solution is removed by acid rinsing steps, followed by the bluing step. The structures of nuclei are more differentiated and better visible by the regressive method. The second staining step is cytoplasmic staining by (Papanicolaou’s solution 2b Orange II solution, (Cat. no. 1106887) or Papanicolaou’s solution 2a Orange G solution (OG6), (Cat. no. 106888)), especially for demonstration of mature and keratinized cells. The target structures are stained orange in different intensities. In the third staining step is used the so-called polychrome solution, a mixture of eosin, light green SF and Bismarck brown, Papanicolaou’s solution 3a polychromatic solution EA 31, (Cat.no 109271), Papanicolaou’s solution 3b polychromatic solution EA 50, (Cat.no. 109272), Papanicolaou’s solution 3c polychromatic solution EA 65,(Cat.no. 109270), or Papanicolaou’s solution 3d polychromatic solution EA 65, (Cat.no.109269). The polychrome solution is used for demonstration of differentiation of squamous cells. Both Papanicolaou’s solution 1a Harris hematoxylin solution(Cat.no. 109253) and Papanicolaou’s solution 1b Hematoxylin solution S (Cat.no. 109254) give a blue to dark violet staining result with clinical specimen material.
          A 500 ml bottle of either solution is sufficient for 1500 -2500 stainings. It is registered as IVD and CE certified product and can be used for clinical diagnostics. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
          References
          Product Information
          HS Code3212 90 90
          Quality LevelMQ400
          Applications
          Applicationfor cytology
          Biological Information
          Physicochemical Information
          Boiling point100 °C (1013 hPa)
          Density1.04 g/cm3 (20 °C)
          pH value2.5 - 3.0 (H₂O, 20 °C)
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Hazard Pictogram(s) Hazard Pictogram(s)
          Hazard Statement(s)H318: Causes serious eye damage.
          EUH210: Safety data sheet available on request.
          Precautionary Statement(s)P280: Wear eye protection/ face protection.
          P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
          Signal WordDanger
          Storage class10 - 13 Other liquids and solids
          WGKWGK 1 slightly hazardous to water
          Disposal28
          Aqueous solutions: Container D.
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          StorageStore at +15°C to +25°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Suitability for microscopy (Vaginal smear)passes test
          Nucleiblue to dark violet
          Cyanophilic Cytoplasm (basophilic)blue-green
          Eosinophilic Cytoplasm (acidophilic)pink
          Global Trade Item Number
          Catalogue Number GTIN
          1.09253.0500 04022536112466
          1.09253.1000 04022536676661

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          Accessories (Primary)

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          Accessories (Secondary)

          Catalogue Number Description  
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          106887 Papanicolaou’s solution 2b Orange II solution Show Pricing & Availability
          106888 Papanicolaou's solution 2a Orange G solution (OG 6) Show Pricing & Availability
          109254 Papanicolaou's solution 1b Hematoxylin solution S Show Pricing & Availability
          109269 Papanicolaou's solution 3d polychromatic solution EA 65 Show Pricing & Availability
          109271 Papanicolaou's solution 3a polychromatic solution EA 31 Show Pricing & Availability
          109272 Papanicolaou's solution 3b polychromatic solution EA 50 Show Pricing & Availability

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          Categories

          IVD/OEM Materials & Reagents > Microscopy > Cytology > Cytological Staining Reagents