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14-584 PAK4 Protein, active, 10 µg

Active, N-terminal His6 & N-terminal GST-tagged, recombinant, human PAK4 amino acids 295-end, for use in Kinase Assays.

PAK4 Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-584
10 µg  Also available in 250 µg size --call for pricing and availability and reference catalog number 14-584M when ordering the 250 µg size.
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      Overview

      Replacement Information
      Description
      Catalogue Number14-584
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPAK4 Protein, active, 10 µg
      Overviewexpressed by baculovirus in Sf21 cells
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, N-terminal His6 & N-terminal GST-tagged, recombinant, human PAK4 amino acids 295-end, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceN-terminal His6 and N-terminal GST-tagged, recombinant, human PAK4 amino acids 295-end
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryPAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
      Gene Symbol
      • PAK4
      • PAK-4
      • KIAA1142
      Protein TargetPAK4
      Purification MethodGlutathione agarose affinity chromatography
      Target Sub-FamilySTE
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O96013 # Activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments.
      SIZE: 591 amino acids; 64072 Da
      SUBUNIT: Interacts with FGFR2 and GRB2 (By similarity). Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and weakly with RAC1.
      TISSUE SPECIFICITY: Highest expression in prostate, testis and colon.
      PTM: Autophosphorylated on serine residues when activated by CDC42/p21. & Phosphorylated on tyrosine residues upon stimulation of FGFR2 (By similarity).
      SIMILARITY: SwissProt: O96013 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. & Contains 1 CRIB domain. & Contains 1 protein kinase domain.
      Molecular Weight61.6kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of MBP (Catalog #13-104)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250 µg size --call for pricing and availability and reference catalog number 14-584M when ordering the 250 µg size.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      14-584 04053252511288

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      14-584M PAK4 Protein, active, 250 µg Show Pricing & Availability

      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases