70181 Sigma-AldrichNovaBlue Singles™ Competent Cells - Novagen
NovaBlue Singles Competent Cells are designed for ultimate convenience and reliability in plasmid transformation.
More>> NovaBlue Singles Competent Cells are designed for ultimate convenience and reliability in plasmid transformation. Less<<NovaBlue Singles™ Competent Cells - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview
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Pricing & Availability
| Catalogue Number | Availability | Packaging | Qty/Pack | Price | Quantity | |
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| 70181-3CN |
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Glass bottle | 11 rxn |
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| 70181-4CN |
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Glass bottle | 22 rxn |
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| Description | |
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| Overview | NovaBlue competent cells are for routine molecular cloning applications. NovaBlue is a K-12 strain derivative that offers high transformation efficiency,
facilitates plasmid stability, and allows blue/white
screening with appropriate plasmids. NovaBlue Singles Competent Cells, like all Novagen Singles Competent Cells, are designed for ultimate convenience and reliability in plasmid transformation. The cells are grown and made chemically competent by an optimized procedure. Cells are provided in 50-µl volumes that eliminate the need to aliquot, freeze/thaw, or waste partially used vials, thus saving time and increasing performance. For use, simply thaw, add DNA, incubate five minutes on ice, heat shock for 30 seconds, place on ice for two minutes, add SOC Medium, and plate directly (when selecting for ampicillin resistance) or after incubation at 37°C for 30 minutes (when selecting for kanamycin resistance). |
| Genotype: endA1 hsdR17 (rK12– mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac F′[proA+B+ lacIqZΔM15::Tn10] (TetR) This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com. |
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| Catalogue Number | 70181 |
| Brand Family | Novagen® |
| References |
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| Product Information | |
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| Components | |
| Genotype | endA1 hsdR17 (rK12– mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac Fʹ[proA+B+ lacIqZΔM15::Tn10] (TetR) |
| Guaranteed efficiency | Guaranteed efficiency: > 1.5 x 10⁸ cfu/µg |
| Quality Level | MQ100 |
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| Safety Information according to GHS |
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| Safety Information |
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| Product Usage Statements |
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| Storage and Shipping Information | |
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| Ship Code | Dry Ice Only |
| Toxicity | Multiple Toxicity Values, refer to MSDS |
| Storage | ≤ -70°C |
| Avoid freeze/thaw | Avoid freeze/thaw |
| Do not freeze | Ok to freeze |
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| Specifications |
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| Global Trade Item Number | |
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| Catalogue Number | GTIN |
| 70181-3CN | 04055977258387 |
| 70181-4CN | 04055977258394 |
Documentation
NovaBlue Singles™ Competent Cells - Novagen SDS
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NovaBlue Singles™ Competent Cells - Novagen Certificates of Analysis
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| 70181 |
Brochure
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| High fidelity gene amplification |
| Protein Expression タンパク質研究ガイド- 発現編 - |
| The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression |
Citations
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User Protocols
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| TB009 Competent Cells |