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70666 Ni-NTA His•Bind® Resin

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70666
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70666-3CN
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      Plastic ampoule 10 ml
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      70666-4CN
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          Glass bottle 25 ml
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          70666-5CN
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              Glass bottle 100 ml
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              Description
              OverviewNi-NTA His•Bind® Resin is a high-performance Ni2+-charged agarose used for rapid one-step purification of proteins containing a His•Tag® sequence by metal chelation chromatography. NTA chemistry minimizes metal leaching during purification and is compatible with up to 10 mM β-mercaptoethanol or 1 mM Tris(hydroxypropyl)phosphine (THP) for reduction of disulfide bonds. Ni-NTA His•Bind® Resin has a binding capacity of 5–10 mg His•Tag® fusion protein per ml resin.

              Supplied as a 50% slurry; the quantity/pack is based on the amount of the settled resin.
              Catalogue Number70666
              Brand Family Novagen®
              References
              Product Information
              Quality LevelMQ400
              Applications
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              R PhraseR: 10-22-42/43

              Flammable.
              Harmful if swallowed.
              May cause sensitization by inhalation and skin contact.
              S PhraseS: 26-36-7

              In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
              Wear suitable protective clothing.
              Keep container tightly closed.
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Shipped at Ambient Temperature or with Blue Ice
              Toxicity Flammable
              Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
              Storage +2°C to +8°C
              Do not freeze Yes
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications
              Global Trade Item Number
              Catalogue Number GTIN
              70666-3CN 07790788054946
              70666-4CN 07790788052768
              70666-5CN 04055977334074

              Documentation

              Ni-NTA His•Bind® Resin SDS

              Title

              Safety Data Sheet (SDS) 

              Ni-NTA His•Bind® Resin Certificates of Analysis

              TitleLot Number
              70666

              Brochure

              Title
              Protein Purification and Detection Tools

              Technical Info

              Title
              Ni-NTA HisユBind® Resins

              Citations

              Title
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            • Wei Cao, et al. (2007) RAET1E2, a soluble isoform of the UL16 binding protein RAET1E produced by tumor cells, inhibits NKG2D-mediated NK cytotoxicity.. Journal of Biological Chemistry 282, 18922-18928.
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            • Ganna Panasyuk, et al. (2006) Nuclear export of S6K1 II is regulated by protein kinase CK2 phosphorylation at ser 17. Journal of Biological Chemistry 281, 31188-31201.
            • Lauren E. Ball, Mary N. Berkaw and Maria G. Buse. (2005) Identification of the major site of O-linked ß-N-acetylglucosamine modification in the C-terminus of insulin receptor substrate-1. Molecular and Cellular Proteomics 5, 313-323.
            • Jeffrey Bethony, et al. (2005) Antibodies against a secreted protein from hookworm larvae reduce the intensity of hookworm infection in humans and vaccinated laboratory animals. Federation of American Societies for Experimental Biology Journal 19, 1743-1745.
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            • Aki Jarvinen, et al. (2005) Metabolic stability of alpha -methylated polyamine derivatives and their use as substitutes for the natural polyamines. Journal of Biological Chemistry 280, 6595-6601.
            • Choong-Min Kang, et al. (2005) The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. Genes and Development 19, 1692-1704.
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            • Nadia Lamour, et al. (2005) Proline metabolism in procyclic Trypanosoma brucei is down-regulated in the presence of glucose. Journal of Biological Chemistry 280, 11902-11910.
            • Maria V. Lara, et al. (2005) NADP-malic enzyme and Hsp70: co-purification of both proteins and modification of NADP-malic enzyme properties by association with Hsp70. Plant and Cell Physiology 46, 997-1006.
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            • Ying Liu, Zhi-Xing Yao and Vassilios Papadopoulos. (2005) Cytochrome p450 17α hydroxylase/17,20 lyase (CYP17) function in cholesterol biosynthesis: identification of squalene monooxygenase (epoxidase) activity associated with CYP17 in Leydig cells. Molecular Endocrinology 19, 1918-1931.
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            • Matthew W. Vetting, et al. (2005) A novel dimeric structure of the rimL N-acetyltransferase from Salmonella typhimurium. Journal of Biological Chemistry 280, 22108-22114.
            • Aileen Aherne, et al. (2004) On the molecular patholo
            • User Protocols

              Title
              TB273 Ni-NTA His•Bind® Resin

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