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101287 Sigma-AldrichLöffler's methylene blue solution

for microscopy

Löffler's methylene blue solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
User Guides

Synonyms: Löfflere'smethylene blue solution, Methylene blue solution acc. to Löffler

View This Product on Sigma-Aldrich

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Overview

Replacement Information

Pricing & Availability

Catalogue Number	Availability	Packaging	Qty/Pack	Price	Quantity
1.01287.0100	Retrieving availability... — Limited Availability In Stock Discontinued Limited Quantities Available Availability to be confirmed Login to See Inventory In Stock Limited Availability In Stock Remaining : Will advise Remaining : Will advise Will advise Contact Customer Service Contact Customer Service Contact Customer Service Contact Customer Service	Plastic bottle	100 ml	Retrieving price... Price could not be retrieved Minimum Quantity is a multiple of Maximum Quantity is Upon Order Completion More Information You Saved () — Request Pricing Login to See Your Pricing	Check Availability —	Add To Favorites Add To Favorites Added To My Favorites
1.01287.0500	Retrieving availability... — Limited Availability In Stock Discontinued Limited Quantities Available Availability to be confirmed Login to See Inventory In Stock Limited Availability In Stock Remaining : Will advise Remaining : Will advise Will advise Contact Customer Service Contact Customer Service Contact Customer Service Contact Customer Service	Plastic bottle	500 ml	Retrieving price... Price could not be retrieved Minimum Quantity is a multiple of Maximum Quantity is Upon Order Completion More Information You Saved () — Request Pricing Login to See Your Pricing	Check Availability —	Add To Favorites Add To Favorites Added To My Favorites

Description
Catalogue Number	101287
Synonyms	Löfflere'smethylene blue solution, Methylene blue solution acc. to Löffler
Description	Löffler's methylene blue solution
Overview	Löffler’s methylene blue solution - for microscopy, is a ready-to-use-solution and is used for human-medical cell diagnosis and serves the purpose of the bacteriological and histological investigation of sample material of human origin, for example smears of enriched bacterial cultures or histological sections of e. g. the lung. The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolate-fuchsin complex in the cell wall. Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use methylene blue is used for counterstaining. Pretreatment of the specimens with Sputofluol® dissolves the bacteria from the surrounding viscid sputum and cell material. The 500 ml bottle is sufficient for approx. 250 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

References

Product Information
HS Code	3212 90 90
Quality Level	MQ400

Applications
Application	for microscopy

Biological Information

Physicochemical Information
Density	0.97 g/cm3 (20 °C)
Flash point	40 °C
pH value	7.0 - 7.5 (H₂O, 20 °C)

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS
Hazard Pictogram(s)
Hazard Statement(s)	H226: Flammable liquid and vapour.
Precautionary Statement(s)	P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. P233: Keep container tightly closed. P240: Ground and bond container and receiving equipment. P241: Use explosion-proof electrical/ ventilating/ lighting equipment. P242: Use non-sparking tools. P243: Take action to prevent static discharges.
Signal Word	Warning
Storage class	3 Flammable liquids
WGK	WGK 1 slightly hazardous to water
Disposal	3 Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

Safety Information
Categories of danger	flammable

Product Usage Statements

Storage and Shipping Information
Storage	Store at +15°C to +25°C.

Packaging Information

Transport Information
Declaration (railroad and road) ADR, RID	UN 1993 , 3, III
Declaration (transport by air) IATA-DGR	UN 1993 , 3, III
Declaration (transport by sea) IMDG-Code	UN 1993 , 3, III

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
1.01287.0100	04022536019024
1.01287.0500	04022536019048

Documentation

Löffler's methylene blue solution SDS

Title
Safety Data Sheet (SDS)

Löffler's methylene blue solution Certificates of Analysis

Product Number	Packaging	Specification	Lot Number
1.01287.0100	Plastic bottle	PDF Specification Document	Enter Lot Number
1.01287.0500	Plastic bottle	PDF Specification Document	Enter Lot Number

User Guides

Title
User Guide (IFU) - 1.01287

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Accessories (Secondary)

Catalogue Number

Description

109215

Ziehl-Neelsen carbol-fuchsin solution