Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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407654
Sigma-AldrichInterleukin-6, Mouse, Recombinant, E. coli
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Description
Overview
Recombinant, mouse interleukin-6 expressed in E. coli. Stimulates the production of acute phase proteins by hepatocytes. Induces growth and differentiation of B cells, hepatocytes, keratinocytes, and nerve cells. Does not inhibit any significant activity on human cells.
Catalogue Number
407654
Brand Family
Calbiochem®
Synonyms
rmIL-6
References
References
Matsuda, T., et al. 1994. Biochem. Biophys. Res. Commun.202, 637.
Product Information
Form
Lyophilized
Formulation
Lyophilized from sterile-filtered 50 mM NaOAc, 1 mM EDTA, 5% trehalose, 50 µg BSA/µg IL-6 pH 4.0.
ED₅₀ = 20-60 pg/ml as measured in a cell proliferation assay with a factor-dependent plasmacytoma cell line
Purity
≥97% by SDS-PAGE
Physicochemical Information
Contaminants
Endotoxin: ≤1 EU/µg IL-6
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Irritant
Storage
≤ -70°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C or -70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 months at -20°C or -70°C or for up to 1 month at 4°C. Avoid freeze/thaw cycles of solutions.
Interleukin-6, Mouse, Recombinant, E. coli Certificates of Analysis
Title
Lot Number
407654
References
Reference overview
Matsuda, T., et al. 1994. Biochem. Biophys. Res. Commun.202, 637.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
08-December-2008 JSW
Synonyms
rmIL-6
Description
Recombinant, mouse interleukin-6 expressed in E. coli. Stimulates the production of acute phase proteins by hepatocytes. Induces growth and differentiation of B cells, hepatocytes, keratinocytes, and nerve cells.
Form
Lyophilized
Formulation
Lyophilized from sterile-filtered 50 mM NaOAc, 1 mM EDTA, 5% trehalose, 50 µg BSA/µg IL-6 pH 4.0.
Purity
≥97% by SDS-PAGE
Contaminants
Endotoxin: ≤1 EU/µg IL-6
Biological activity
ED₅₀ = 20-60 pg/ml as measured in a cell proliferation assay with a factor-dependent plasmacytoma cell line
Solubility
Reconstitute to a concentration of ≥10 µg/ml with sterile PBS containing at least 0.1% HSA or BSA.
Storage
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C or -70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 months at -20°C or -70°C or for up to 1 month at 4°C. Avoid freeze/thaw cycles of solutions.
Toxicity
Irritant
References
Matsuda, T., et al. 1994. Biochem. Biophys. Res. Commun.202, 637.
