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IPFL00010 Immobilon®-FL PVDF Membrane

1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

Immobilon®-FL PVDF Membrane MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Western Blotting, Dot Blot
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IPFL00010
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      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      WB, DB
      Description
      Catalogue NumberIPFL00010
      Trade Name
      • Immobilon
      DescriptionImmobilon®-FL PVDF Membrane
      Background InformationOverview
      - Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
      Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

      Immobilon-P Transfer Membrane:
      - 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
      - Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

      Immobilon-E Membrane:
      - the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
      - 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

      Blotting Sandwiches:
      - When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

      Blotting Filter Paper:
      - Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

      Immobilon-PSQ Membrane:
      - 0.2 µm pore size and a large internal structure
      - Higher protein adsorption and sequencing yields than other membranes
      - Recommended for blotting proteins less than 20 kDa
      - Prevents blow-through of low molecular weight proteins

      Immobilon-FL Membrane:
      - The first transfer membrane optimized for fluorescence applications
      - Extremely low background improves sensitivity of all fluorescence detection protocols
      - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
      - Ideal for multiplexing and chemifluorescence applications

      Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

      Features & Benefits:
      - Won’t crack, curl or fracture when cut
      - Low background
      - Superior staining capabilities
      - Can be reprobed multiple times

      Applications:
      - Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
      - Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
      - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
      References
      Product Information
      Detection methodFluorescent Chemifluorescence Chromogenic Chemiluminescence 125I Fluorescent
      Compatible Stains
      • All fluorescence dyes
      • Coomassie brilliant blue
      • Amido black
      • Ponceau-S red
      • CPTS
      • Sypro® ruby
      Filter CodeIPFL
      Filter ColorWhite
      Quality LevelMQ400
      Applications
      Application1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
      Key Applications
      • Western Blotting
      • Dot Blot
      Biological Information
      BSA Adsorption205 µg/cm²
      Goat IgG Adsorption300 µg/cm²
      Insulin Adsorption155 µg/cm²
      WettabilityHydrophobic
      Physicochemical Information
      Pore Size0.45 µm
      Dimensions
      Filter SurfacePlain
      Filter Dimensions27 cm x 3.75 m
      Materials Information
      Chemistry
      • Hydrophobic Polyvinylidene Fluoride (PVDF)
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Packaging Information
      Material Size1
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      IPFL00010 04053252669439

      Documentation

      Immobilon®-FL PVDF Membrane SDS

      Title

      Safety Data Sheet (SDS) 

      Brochure

      Title
      SNAP i.d. 2.0 System Brochure
      Western Blotting Tools

      Technical Info

      Title
      Antibody recovery and reuse in the SNAP i.d.®2.0 immunodetection system
      Protein Blotting Handbook: 6th Edition

      Posters

      Title
      Low Background Membrane for Fluorescent Protein Detection in Western Blotting

      User Guides

      Title
      Immobilon® Block Noise Cancelling Reagents
      Immobilon®-FL Transfer Membrane User Guide
      User Guide - Immobilon® FL Transfer Membrane