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407255 IGFBP-rp1/IGFBP-7, His-Tag, CF, Mouse, Recombinant

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407255
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Overview

Replacement Information

Key Spec Table

Purity
≥97% by SDS-PAGE

Pricing & Availability

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407255-25UGCN
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      Glass bottle 25 μg
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      Description
      OverviewRecombinant, mouse insulin-like growth factor binding protein-related protein 1 (IGFBP-rp1) consisting of amino acids 28-281 and expressed in S. frugiperda 21 cells with an N-terminal His•Tag®. Mouse IGFBP-rp1 cDNA encodes a 281 amino acid precursor protein which contains a putative 25 aa signal peptide and a 256 aa mature region. IGFBP modifies affinities of the binding proteins to IGF.
      Catalogue Number407255
      Brand Family Calbiochem®
      SynonymsIGFBP-7
      References
      ReferencesAhmed, S. et al. 2003. Biochem. Biophys. Res. Commun. 310, 612.
      Nagakubo, D. et al. 2003. J. Immunol. 171, 553.
      Wilson, HM. et al. 2002. Cell Growth Differ. 13, 205.
      Degeorges, A. et al. 2000. J. Histochem. Cytochem. 48, 747.
      Kato, M.V. 2000. Mol. Med. 6, 126.
      Hwa, V. et al. 1999. Endocr. Rev. 20, 761.
      Kim, HS. et al. 1997. Proc. Natl. Acad. Sci. USA 94,12981.
      Kato, M.V. et al. 1996. Oncogene 12, 1361.
      Oh, Y. et al. 1996. J. Biol. Chem. 271, 30322.
      Product Information
      FormSolid
      FormulationLyophilized from 0.2 µm sterile-filtered PBS.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityFunctional ELISA Immobilized rmIGFBP-rp1/IGFBP-7 (500 ng/ml) produces 50% optimal binding to rhCCL21/6Ckine at 4-20 ng/ml.
      Purity≥97% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: <1.0 EU per/1 µg protein
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      407255-25UGCN 04055977188738

      Documentation

      IGFBP-rp1/IGFBP-7, His-Tag, CF, Mouse, Recombinant SDS

      Title

      Safety Data Sheet (SDS) 

      IGFBP-rp1/IGFBP-7, His-Tag, CF, Mouse, Recombinant Certificates of Analysis

      TitleLot Number
      407255

      References

      Reference overview
      Ahmed, S. et al. 2003. Biochem. Biophys. Res. Commun. 310, 612.
      Nagakubo, D. et al. 2003. J. Immunol. 171, 553.
      Wilson, HM. et al. 2002. Cell Growth Differ. 13, 205.
      Degeorges, A. et al. 2000. J. Histochem. Cytochem. 48, 747.
      Kato, M.V. 2000. Mol. Med. 6, 126.
      Hwa, V. et al. 1999. Endocr. Rev. 20, 761.
      Kim, HS. et al. 1997. Proc. Natl. Acad. Sci. USA 94,12981.
      Kato, M.V. et al. 1996. Oncogene 12, 1361.
      Oh, Y. et al. 1996. J. Biol. Chem. 271, 30322.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision21-Sepmtember-2011 RFH
      SynonymsIGFBP-7
      DescriptionRecombinant, mouse insulin-like growth factor binding protein-related protein 1 (IGFBP-rp1) consisting of amino acids 28-281 and expressed in S. frugiperda 21 cells with an N-terminal His•Tag®. Mouse IGFBP-rp1 cDNA encodes a 281 amino acid precursor protein which contains a putative 25 aa signal peptide and a 256 aa mature region. IGFBP modifies affinities of the binding proteins to IGF.
      FormSolid
      FormulationLyophilized from 0.2 µm sterile-filtered PBS.
      Purity≥97% by SDS-PAGE
      ContaminantsEndotoxin: <1.0 EU per/1 µg protein
      Biological activityFunctional ELISA Immobilized rmIGFBP-rp1/IGFBP-7 (500 ng/ml) produces 50% optimal binding to rhCCL21/6Ckine at 4-20 ng/ml.
      SolubilityReconstitute to 100 µg/ml in sterile PBS.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesAhmed, S. et al. 2003. Biochem. Biophys. Res. Commun. 310, 612.
      Nagakubo, D. et al. 2003. J. Immunol. 171, 553.
      Wilson, HM. et al. 2002. Cell Growth Differ. 13, 205.
      Degeorges, A. et al. 2000. J. Histochem. Cytochem. 48, 747.
      Kato, M.V. 2000. Mol. Med. 6, 126.
      Hwa, V. et al. 1999. Endocr. Rev. 20, 761.
      Kim, HS. et al. 1997. Proc. Natl. Acad. Sci. USA 94,12981.
      Kato, M.V. et al. 1996. Oncogene 12, 1361.
      Oh, Y. et al. 1996. J. Biol. Chem. 271, 30322.