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109218 Gram's crystal violet solution

for the Gram staining method

Gram's crystal violet solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
109218
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Overview

Replacement Information

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1.09218.0500
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      		 Plastic bottle 500 ml
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      Description
      Catalogue Number109218
      DescriptionGram's crystal violet solution
      OverviewGram's crystal violet solution for the Gram staining method, is a ready-to-use solution used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative.The mureine structure of the bacteria wall is the basis of the color affinity. In the first step, bacteria will be stained with Gram's crystal violet solution (Cat. No.109218), an aniline dye. After the treatment with iodine solution (Lugol’s solution stabilized with PVP, Cat No.100567), a dye-iodine complex will form. During the decolorizing step (Gram’s decolorizing solution, Cat. No. 110218), this complex stays in the multilayer mureine structure of the cell wall of Gram-positive bacteria - they will appear blue-violet. Gram-negative bacteria, by contrast, have a cell wall consisting of a single-layered murein structure, and correspondingly re-release the staining dye with the decoloring solution. Gram-negative bacteria will be counterstained with safranine solution (Gram's safranine solution (Cat.no. 109217) and will then appear pink to red.
      A bottle with 500 ml Gram's crystal violet solution is sufficient for staining up to 250 slides. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
      References
      Product Information
      HS Code3212 90 90
      Quality LevelMQ400
      Applications
      Applicationfor the Gram staining method
      Biological Information
      Physicochemical Information
      Density0.99 g/cm3 (20 °C)
      Flash point47 °C
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Hazard Pictogram(s) Hazard Pictogram(s)
      Hazard Statement(s)H226: Flammable liquid and vapour.
      H412: Harmful to aquatic life with long lasting effects.
      Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
      P233: Keep container tightly closed.
      P240: Ground and bond container and receiving equipment.
      P241: Use explosion-proof electrical/ ventilating/ lighting equipment.
      P242: Use non-sparking tools.
      P273: Avoid release to the environment.
      Signal WordWarning
      Storage class3 Flammable liquids
      WGKWGK 2 obviously hazardous to water
      Disposal28
      Aqueous solutions: Container D.
      Safety Information
      Categories of dangerflammable, dangerous for the environment
      Product Usage Statements
      Storage and Shipping Information
      StorageStore at +15°C to +25°C.
      Packaging Information
      Transport Information
      Declaration (railroad and road) ADR, RIDKein Gefahrgut
      Declaration (transport by air) IATA-DGRNo Dangerous Good
      Declaration (transport by sea) IMDG-CodeNo Dangerous Good
      Supplemental Information
      Specifications
      Suitability for microscopypasses test
      gram-positve microorganismsblue-violet
      gram-negative microorganismspink to red
      Global Trade Item Number
      Catalogue Number GTIN
      1.09218.0500 04022536112077

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      Accessories (Primary)

      Catalogue Number Description  
      100567 Lugol's solution Show Pricing & Availability
      109217 Gram's safranine solution Show Pricing & Availability
      109261 Lugol's solution (diluted iodine-potassium iodide solution) Show Pricing & Availability
      110218 Gram's decolorizing solution Show Pricing & Availability

      Accessories (Secondary)

      Catalogue Number Description  
      100567 Lugol's solution Show Pricing & Availability
      109217 Gram's safranine solution Show Pricing & Availability
      109261 Lugol's solution (diluted iodine-potassium iodide solution) Show Pricing & Availability
      111885 Gram-Color Show Pricing & Availability

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      Categories

      IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Gram-Bacteria Staining