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AP502B Goat Anti-Human Ig κ chain Antibody, biotin conjugate, Species Adsorbed

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AP502B
1 mg  
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      Overview

      Description
      Catalogue NumberAP502B
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionGoat Anti-Human Ig κ chain Antibody, biotin conjugate, Species Adsorbed
      Background InformationAntibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and lambda light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.
      Product Information
      FormatBiotin
      PresentationPurified by affinity chromatography on pooled human Igs with κ light chains covalently linked to agarose. Liquid in 2.0 mL PBS/NaN3.
      Quality LevelMQ100
      Applications
      ApplicationThis Goat anti-Human Ig κ chain Antibody, biotin conjugate, Species Adsorbed is validated for use in ELISA, IF for the detection of Human Ig κ chain.
      Key Applications
      • ELISA
      • Immunofluorescence
      Application NotesImmunofluorescence: ≤ 1 μg/106 cells.
      ELISA: 1:5,000-1:20,000.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPrepared from pooled antisera from goats hyperimmunized with human myeloma proteins with κ light chains.
      EpitopeIg kappa chain
      Concentration0.5 mg/mL
      HostGoat
      SpecificityThe antibody reacts with human kappa light chains as demonstrated by ELISA and flow cytometry.
      Species Reactivity
      • Human
      Species Reactivity NoteReacts with human kappa light chains. Absorbed for human myeloma proteins with λ light chains and mouse immunoglobulins.
      Antibody TypePolyclonal Antibody
      Purification MethodAffinity Chromatography
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt.
      Packaging Information
      Material Size1 mg
      Global Trade Item Number
      Catalogue Number GTIN
      AP502B 04053252335549