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PMEF-CFL-P1 EmbryoMax® Primary Mouse Embryonic Fibroblasts

PMEF, Strain CF1, Not Treated, Passage 1

EmbryoMax® Primary Mouse Embryonic Fibroblasts MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture
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PMEF-CFL-P1
1 vial  1x106 cells
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      Key Spec Table

      Key Applications
      Cult
      Description
      Catalogue NumberPMEF-CFL-P1
      Trade Name
      • Embryomax
      • Specialty Media
      DescriptionEmbryoMax® Primary Mouse Embryonic Fibroblasts
      OverviewPMEF-CFL-P1 are low passage Primary Mouse Embryo Fibroblasts (PMEFs) isolated from CF1 mouse embryos at day 13. The cells have NOT been mitotically arrested. PMEFs are the traditional feeder cells used to support the growth of undifferentiated mouse and human pluripotent stem cell cultures. Additionally, low passage primary cultures of fibroblasts have a high proliferative capacity and can be used for reprogramming. It is known that cellular reprogramming efficiency declines with each passage, presumably due to lowered proliferative capacity of PMEFs at later passages. These untreated PMEF cells are high quality, low passage (P1) mouse fibroblasts that have been successfully reprogrammed into iPS cells with high efficiency. They can be used to study the mechanisms of reprogramming or be further expanded and treated with γ-irradiation or mitomycin-C to make growth arrested feeder cells. See Murine Embryonic Stem Cell Culture Manual for complete culture and media for PMEF cells.
      Alternate Names
      • MEF Feeder Cells, CF-1 PMEFs, CF-1 MEFs, MEFs, Mouse Feeder Cells
      Background InformationThe EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.
      References
      Product Information
      PresentationProvided frozen in EMD Millipore Freezing Medium (S-002-D) containing DMSO.
      Quality LevelMQ100
      Applications
      ApplicationPMEF, Strain CF1, Not Treated, Passage 1
      Key Applications
      • Stem Cell Culture
      Biological Information
      Stem Cell Type
      • Mouse Embryonic Stem Cells
      • PMEFs
      • Induced Pluripotent Stem Cells
      Cell Line Type
      • Primary Mouse Embryo Fibroblasts
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsAfter receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.
      Packaging Information
      Material Size1 vial
      Material Package1x106 cells
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PMEF-CFL-P1 04053252851711

      Documentation

      EmbryoMax® Primary Mouse Embryonic Fibroblasts SDS

      Title

      Safety Data Sheet (SDS) 

      EmbryoMax® Primary Mouse Embryonic Fibroblasts Certificates of Analysis

      TitleLot Number
      EmbryoMax® PMEF P1 Strain CF-1, Untreated - 2389219 2389219
      EmbryoMax® PMEF P1 -2492810 2492810
      EmbryoMax® PMEF P1 Strain CF-1, Untreated - 3133227 3133227
      EmbryoMax® PMEF P1 Strain CF-1, Untreated - 3153783 3153783
      EmbryoMax® PMEF P1 Strain CF-1, Untreated - 3178415 3178415
      EmbryoMax® PMEF P1 Strain CF-1, Untreated - 3438076 3438076
      EmbryoMax® PMEF P1 Strain CF-1, Untreated - 3681329 3681329
      EmbryoMax® PMEF P1 Strain CF-1, Untreated - 3796896 3796896
      EmbryoMax® PMEF P1 Strain CF-1, Untreated - 3801061 3801061
      EmbryoMax® PMEF P1 Strain CF-1, Untreated - 3924980 3924980
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      PMEF-CFL EmbryoMax® Primary Mouse Embryonic Fibroblasts Show Pricing & Availability

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      Categories

      Life Science Research > Cell Culture and Systems > Cell Lines > Primary Mouse Embryo Fibroblast (PMEF) Feeder Cells