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SF006 ESGRO Complete Accutase

The ESGRO Complete Accutase is a cell detachment solution of proteolytic & collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium.

ESGRO Complete Accutase MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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SF006
100 mL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSF006
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionESGRO Complete Accutase
      OverviewESGRO Complete™ Accutase™ is a cell detachment solution of proteolytic and collagenolytic enzymes that has been qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete™ Clonal Grade Medium (Cat. No. SF001-500). Accutase™ does not contain mammalian or bacterial derived products.
      References
      Product Information
      HS Code3821 00 00
      PresentationFrozen sterile filtered (0.2 micron) liquid, ready to use formulation. Each lot is tested for Sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.

      1X Accutase™ enzymes in Dulbecco's PBS containing 0.5 mM EDTA•4Na and 3 mg/L Phenol Red.
      Quality LevelMQ100
      Applications
      ApplicationThe ESGRO Complete Accutase is a cell detachment solution of proteolytic & collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium.
      Key Applications
      • Stem Cell Culture
      Application NotesCell Detachment:

      1. Thaw Accutase™ to room temperature.

      2. Wash plate, flask or beads with sterile PBS.

      3. Add Accutase™ to culture dish or flask using aseptic procedures at 10 mL per 75 cm2 surface area.

      4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes).

      5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required.
      Biological Information
      Media FormLiquid
      Stem Cell Type
      • Mouse Embryonic Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable when stored at -20°C. Refer to lot expiration date on label. Recommended storage upon receipt is -20°C. After thawing, Accutase™ may be stored for up to 2 months at 4°C. DO NOT STORE AT ROOM TEMPERATURE.
      Packaging Information
      Material Size100 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SF006 04053252503061

      Documentation

      Protocols

      Title
      Feeder-free and Serum-free ES Cell Culture using ESGRO Complete System

      ESGRO Complete Accutase SDS

      Title

      Safety Data Sheet (SDS) 

      ESGRO Complete Accutase Certificates of Analysis

      TitleLot Number
      ESGRO COMPLETE™ ACCUTASE™ - 2300854 2300854

      References

      Reference overviewPub Med ID
      Efficient generation of germ line transmitting chimeras from C57BL/6N ES cells by aggregation with outbred host embryos.
      Gertsenstein M. et al.
      PLoS One,  5(6)  e11260  2010

      Show Abstract       		20582321 20582321

      Brochure

      Title
      Murine Embryonic Stem Cell Culture Procedures & Protocols

      Technical Info

      Title
      Derivation of Mouse Embryonic Stem Cells in Serum-Free and Feeder-Free Conditions
      ESGRO Complete Clonal Grade Medium: Serum-Free Medium for Mouse Embryonic Stem Cell Culture
      ESGRO Complete Products for Mouse Embryonic Stem Cell Culture in Serum-Free and Feeder-Free Conditions
      The Genetically Modified Mouse: An Indispensable Tool for Disease Modeling

      Data Sheet

      Title
      ESGRO COMPLETE™ ACCUTASE™