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100591 Sigma-AldrichELASTIN staining solution acc. to Weigert

for microscopy

ELASTIN staining solution acc. to Weigert MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
User Guides

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Overview

Replacement Information

Pricing & Availability

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Description
Catalogue Number	100591
Description	ELASTIN staining solution acc. to Weigert
Overview	The ELASTIN staining solution acc. to Weigert - for microscopy, is a ready-to-use solution used to detect elastic fibers in connective tissues. Elastic connective-tissue fibers are bundles of glycoprotein-containing microfibrils that accumulate the polymeric protein elastin in their development, gradually resulting in the formation of coarse elastic fibers and membranes. The microfibrils on the surface of the elastic connective-tissue fibers expose high numbers of disulfide groups, which can be chemically detected when sufficient material is available for light-microscopic investigations. Furthermore, they are strongly acidic and thus bind the resorcin-fuchsin dye by electropolarity via interface adsorption. The resorcin-fuchsin staining method is a regressive method. In tissue sections, nuclei can be stained using a 0.1% nuclear fast red-aluminum sulfate solution (Cat.no. 100121) if not only elastic fibers, but also nuclei and the background also need to be visualized. Brilliant staining results of these fibres are usually achieved when using the ELASTIN staining solution acc. to Weigert - for microscopy in combination with the Picrofuchsin solution acc. van Gieson - for microscopy, Cat. No. 100199 and nuclear staining with Weigert’s iron hematoxylin kit - for nuclear staining in histology, Cat. No. 115973 respectively Nuclear fast red-aluminium sulfate solution 0.1% - for microscopy, Cat. No. 100121. The 500ml bottle is sufficient for 400 - 500 applications. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

References

Product Information
HS Code	3212 90 00
Quality Level	MQ400

Applications
Application	for microscopy

Biological Information

Physicochemical Information
Boiling point	78.3 °C (1013 hPa) (ethanol)
Density	0.84 g/cm3 (20 °C)
Explosion limit	3.1 - 27.7 %(V) (ethanol)
Flash point	18 °C
Vapor pressure	59 hPa (20 °C)

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS
Hazard Pictogram(s)
Hazard Statement(s)	H225: Highly flammable liquid and vapour. H290: May be corrosive to metals. H317: May cause an allergic skin reaction. H319: Causes serious eye irritation. H351: Suspected of causing cancer. H371: May cause damage to organs.
Precautionary Statement(s)	P202: Do not handle until all safety precautions have been read and understood. P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection. P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water. P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P308 + P311: IF exposed or concerned: Call a POISON CENTER/doctor. P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
Signal Word	Danger
Storage class	3 Flammable liquids
WGK	WGK 3 highly hazardous to water

Safety Information
Categories of danger	highly flammable

Product Usage Statements

Storage and Shipping Information
Storage	Store at +2°C to +8°C.

Packaging Information

Transport Information
Declaration (railroad and road) ADR, RID	UN 2924 , 3 (8), II
Declaration (transport by air) IATA-DGR	UN 2924 , 3 (8), II
Declaration (transport by sea) IMDG-Code	UN 2924 , 3 (8), II, Segregation Group: 1 (Acids)

Supplemental Information

Specifications
Suitability for microscopy	passes test
Nuclei	blue-brown
Collagen	red
Muscle fibers	yellow
Cytoplasm	yellow
Elastic fibers	black

Global Trade Item Number
Catalogue Number	GTIN
1.00591.0500	04027269377861

Documentation

ELASTIN staining solution acc. to Weigert SDS

Title
Safety Data Sheet (SDS)

ELASTIN staining solution acc. to Weigert Certificates of Analysis

Product Number	Packaging	Specification	Lot Number
1.00591.0500	Plastic bottle	PDF Specification Document	Enter Lot Number

User Guides

Title
User Guide (IFU) - 1.00591

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Description

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Accessories (Primary)

Catalogue Number

Description

115973

Weigert's iron hematoxylin kit