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251520 DAF-FM DA - CAS 254109-22-3 - Calbiochem

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.

DAF-FM DA - CAS 254109-22-3 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate

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251520
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
254109-22-3C₂₅H₁₈F₂N₂O₇

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      Description
      OverviewA cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Intracellularly releases DAF-FM (Cat. No. 251515) by the action of esterases. Reacts with NO, in the presence of O2, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence is reported to be stable above pH 5.8.
      Catalogue Number251520
      Brand Family Calbiochem®
      Synonyms3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate
      References
      ReferencesKojima, H., et al. 2001. J. Neurochem. 76, 1404.
      Park. J.M., et al. 2001. Br. J. Pharmacol. 132, 1876.
      Itoh, Y., et al. 2000. Anal. Biochem. 287, 203.
      Kojima, H., et al. 1999. Angew Chem. Int. Ed. 38, 3209.
      Product Information
      CAS number254109-22-3
      ATP CompetitiveN
      DeclarationManufactured by Daiichi Pure Chemicals Co., Ltd
      FormPale yellow solution
      FormulationA 5 mM solution in DMSO.
      Hill FormulaC₂₅H₁₈F₂N₂O₇
      Chemical formulaC₂₅H₁₈F₂N₂O₇
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetNitric oxide (NO) fluorescent indicator
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableY
      Emission max.
      Excitation max.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/38

      Irritating to eyes and skin.
      S PhraseS: 26

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Irritant
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      251520-1MGCN 04055977198515

      Documentation

      DAF-FM DA - CAS 254109-22-3 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      DAF-FM DA - CAS 254109-22-3 - Calbiochem Certificates of Analysis

      TitleLot Number
      251520

      References

      Reference overview
      Kojima, H., et al. 2001. J. Neurochem. 76, 1404.
      Park. J.M., et al. 2001. Br. J. Pharmacol. 132, 1876.
      Itoh, Y., et al. 2000. Anal. Biochem. 287, 203.
      Kojima, H., et al. 1999. Angew Chem. Int. Ed. 38, 3209.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-December-2018 JSW
      Synonyms3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate
      DescriptionA cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Releases DAF-FM (Cat. No. 251515) by the action of intracellular esterases. Reacts with NO, in the presence of oxygen, resulting in the formation of a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8. Excitation max.: ~500 nm; emission max.: ~515 nm.
      FormPale yellow solution
      FormulationA 5 mM solution in DMSO.
      Recommended reaction conditions
      The reagent is provided at a 5 mM concentration. Prepare a 500-fold dilution in 0.1 M phosphate buffer, pH 7.4 before use. BSA and phenol red may affect fluorescence.
      CAS number254109-22-3
      Chemical formulaC₂₅H₁₈F₂N₂O₇
      Structure formulaStructure formula
      Purity≥98% by HPLC
      Storage Protect from light
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.
      Toxicity Irritant
      ReferencesKojima, H., et al. 2001. J. Neurochem. 76, 1404.
      Park. J.M., et al. 2001. Br. J. Pharmacol. 132, 1876.
      Itoh, Y., et al. 2000. Anal. Biochem. 287, 203.
      Kojima, H., et al. 1999. Angew Chem. Int. Ed. 38, 3209.