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SCC428 D4M-3A Mouse Melanoma Cell Line

The D4M-3A cell line is a clinically relevant model for both in vitro and in vivo studies of metastatic melanoma

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SCC428
≥1X10⁶ cells/vial   
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCC428
      DescriptionD4M-3A Mouse Melanoma Cell Line
      Alternate Names

      • Dartmouth murine mutant malignant melanoma-3A
      Background InformationMelanoma is one of the most prevalent human cancers, affecting 1 out of every 50 individuals in their lifetime.1 The BRafV600E mutation, which occurs in nearly 50% in human melanomas, constitutively activates pERK, a component of the MAP kinase signaling pathway, and contributes to disease progression.2 Although human melanoma cell lines harboring the BRafV600E mutation are well-established, the reliance on xenografts in immunocompromised mice limits studies of host-tumor cell interactions in a syngeneic background.2
      The D4M-3A cell line is a melanoma cell line from the conditional mouse model of metastatic melanoma: Tyr::CreER, BrafCA;Ptenlox/lox, which recapitulates human disease. Cultured D4M-3A cells express high constitutive pERK and respond to the BRafV600E inhibitor, Vemurafenib (PLX4032).2 D4M-3A cells lack melanin expression but maintain ability to express melanocyte differentiation antigens, which are important immune recognition factors. D4M-3A cells are transplantable in either immune-compromised or syngeneic B6 mice and show correlation of in vitro studies on molecular mechanisms of melanoma with in vivo investigations on pathology and immunology. The D4M-3A cell line is a clinically relevant model for both in vitro and in vivo studies of metastatic melanoma.

      Source
      The D4M-3A cell line was derived from a male transgenic Braf/Pten mouse.2

      References
      1. CA Cancer J Clin 2004, 54(3): 131-149.
      2. Pigma Cell Melanoma Res 2014, 27(3): 495-501
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationThe D4M-3A cell line is a clinically relevant model for both in vitro and in vivo studies of metastatic melanoma
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Cancer Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEach vial contains ≥1X10⁶ viable cells.

      Cells are tested negative for infectious diseases by a Mouse Essential CLEAR Panel by Charles River Animal Diagnostic Services.

      Cells are verified to be of mouse origin and negative for inter-species contamination from rat, human, chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services

      Cells are negative for mycoplasma contamination.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Storage and Shipping Information
      Storage ConditionsThe cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCC428 04065266920116