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17-10199 ChIPAb+™ HDAC1 Antibody, rabbit polyclonal

This ChIPAb+ HDAC1 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

ChIPAb+™ HDAC1 Antibody, rabbit polyclonal MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Western Blotting, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP) Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones
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17-10199
25 assays  25 assays per set. Recommended use: ~2 μg of antibody per chromatin immunoprecipitation (dependent upon biological context).
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      Key Spec Table

      Species ReactivityKey Applications
      M, H, R, Ca, HtWB, ICC, ChIP
      Description
      Catalogue Number17-10199
      Trade Name
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+™ HDAC1 Antibody, rabbit polyclonal
      OverviewAll ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
      The ChIPAb+ HDAC1 polyclonal set includes the HDAC1 antibody, a Normal Rabbit IgG, and control primers which amplify a 88 bp region of ChIP Primers p21. The HDAC1 and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of HDAC1-associated chromatin.
      References
      Product Information
      FormatPurified
      Control
      • Includes normal rabbit IgG and primers specific for human p21.
      PresentationAnti-HDAC1 (rabbit polyclonal). One vial containing 50 µg of Anti-HDAC1 IgG in PBS with 1 mg/mL BSA and ≤0.1% sodium azide before the addition of glycerol to 30%. Store at -20°C.
      Concentration: 0.7 mg/mL
      Normal Rabbit IgG. One vial containing 125 µg of rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C.
      ChIP Primers p21. One vial containing 75 μL of 5 μM of each primer specific for a region of the human p21 (WAF1/CIP1/CDKN1A) promoter. Store at -20°C.
      FOR: CCC ACA GCA GAG GAG AAA GAA
      REV: CTG GAA ATC TCT GCC CAG ACA
      Quality LevelMQ100
      Applications
      ApplicationThis ChIPAb+ HDAC1 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation:
      Representative lot data.
      Sonicated chromatin prepared from untreated or UV treated (6 hrs, 50 joules/m2.) U2OS cells (3 X 10E6 cell equivalents per IP) was subjected to chromatin immunoprecipitation using using 2 µg of either Normal Rabbit IgG, or 2 µg of Anti-HDAC1 and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of HDAC1 associated DNA fragments was verified by qPCR using ChIP Primers, p21 . (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
      Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
      Western Blot Analysis:
      Representative lot data.
      0.5 µg/mL from a representative lot detected HDAC1 in HeLa cells. (Figure 3).

      Immunofluorescence Analysis:
      2 µg/mL from a representative lot detected HDAC1 in HeLa cells.
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 467-482 of human HDAC1.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Mouse
      • Human
      • Rat
      • Canine
      • Hamster
      Species Reactivity NoteDemonstrated to react with human. Predicted to react with rat, mouse, canine, and hamster based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Molecular Weight~62 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceChromatin Immunoprecipitation:
      Representative lot data.
      Sonicated chromatin prepared from U2OS cells (3 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG, or 2 µg of Anti-HDAC1 and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of HDAC1 associated DNA fragments was verified by qPCR using ChIP Primers, p21 flanking an Sp1 binding site in the human p21 promoter (Figure 1).
      Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size25 assays
      Material Package25 assays per set. Recommended use: ~2 μg of antibody per chromatin immunoprecipitation (dependent upon biological context).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      17-10199 04053252296628

      Documentation

      ChIPAb+™ HDAC1 Antibody, rabbit polyclonal SDS

      Title

      Safety Data Sheet (SDS) 

      ChIPAb+™ HDAC1 Antibody, rabbit polyclonal Certificates of Analysis

      TitleLot Number
      ChIPAb+ HDAC1 - 2053033 2053033
      ChIPAb+ HDAC1 - Q1922566 Q1922566
      ChIPAb+™ HDAC1 - 2561140 2561140
      ChIPAb+™ HDAC1 - 3383260 3383260
      ChIPAb+™ HDAC1 - 3598089 3598089
      ChIPAb+™ HDAC1 - 3698772 3698772
      ChIPAb+™ HDAC1 - 3924105 3924105
      ChIPAb+™ HDAC1 - 4155314 4155314
      ChIPAb+™ HDAC1 -2584062 2584062
      ChIPAb+™ HDAC1 -2611211 2611211
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      Brochure

      Title
      Advance your Epigenetics Research
      New Products: Volume 3, 2012