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14-450M Cdk1/cyclin B Protein, active, 250 µg

Active, C-terminal His6-tagged human full length Cdk1 & N-terminal GST-tagged human full length Cyclin B, for use in Kinase Assays.

Cdk1/cyclin B Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-450M
250 µg  Also available in 10 μg size (cat#14-450) and in bulk (cat# 14-450-K).
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      Overview

      Replacement Information
      Description
      Catalogue Number14-450M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionCdk1/cyclin B Protein, active, 250 µg
      OverviewC-terminal His6-tagged human full length cdk1, and N-terminal GST-tagged human full length cyclin B
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, C-terminal His6-tagged human full length Cdk1 & N-terminal GST-tagged human full length Cyclin B, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourceexpressed individually by baculovirus in Sf21 insect cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.
      Gene Symbol
      • CDC2
      • CDK1
      • MGC111195
      • DKFZp686L20222
      • CDC28A
      Protein TargetCDK1/cyclinB
      Purification MethodPurified using Ni2+/NTA-agarose and GST-agarose, respectively. The cdk1 is then activated using CAK and repurified by Q Sepharose™ and Ni2+/NTA-agarose
      Target Sub-FamilyCMGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P06493 # Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
      SIZE: 297 amino acids; 34095 Da
      SUBUNIT: Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with DLG7. Isoform 2 is unable to complex with cyclin B1 and also fails to bind to the CDK inhibitor p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase.
      SUBCELLULAR LOCATION: Nucleus (By similarity).
      SIMILARITY: SwissProt: P06493 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
      Molecular Weight35kDa (cdk1) and 75kDa (cyclin B)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of the Histone H1 substrate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-450) and in bulk (cat# 14-450-K).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      14-450M 04053252283772

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      14-450 Cdk1/cyclin B Protein, active, 10 µg Show Pricing & Availability

      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases