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219362 Cathepsin B, Human Liver

Cathepsin B, Human Liver, CAS 9047-22-7, is a purified native cathepsin B from human liver, purified by affinity chromatography. Upregulated in many types of tumors.

Cathepsin B, Human Liver MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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219362
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Overview

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219362-50UGCN
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      		 Plastic ampoule 50 μg
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      Description
      OverviewNative cathepsin B from human liver, purified by affinity chromatography and HPLC. The most investigated enzyme of all lysosomal cysteine proteases. Upregulation and secretion of cathepsin B have been shown to occur in many types of tumors and to correlate positively with their invasive and metastatic capabilities. Cathepsin B is shown to facilitate tumor invasion by dissolving extracellular barriers.
      Catalogue Number219362
      Brand Family Calbiochem®
      References
      ReferencesHirai, K., et al. 1999. Hum. Pathol. 30, 680.
      Kostoulas, G., et al. 1999. FEBS Lett. 455, 286.
      Strojnik, T., et al. 1999. Clin. Cancer Res. 5, 559.
      Maquire, T.M., et al. 1998. Int. J. Biol. Markers 13, 139.
      Berquim, I.M., and Sloane, B.F. 1996. Adv. Exp. Med. Biol. 389, 281.
      Product Information
      CAS number9047-22-7
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-RR-β-naphthylamide per min at 40°C, using 100 mM Na<sup>+</sup>/K<sup>+</sup> pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.
      EC number3.4.22.1
      FormLiquid
      FormulationIn 50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by SDS-PAGE
      SourcePrepared from tissues of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      Specific Activity≥10 units/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      219362-50UGCN 04055977202205

      Documentation

      Cathepsin B, Human Liver SDS

      Title

      Safety Data Sheet (SDS) 

      Cathepsin B, Human Liver Certificates of Analysis

      TitleLot Number
      219362

      References

      Reference overview
      Hirai, K., et al. 1999. Hum. Pathol. 30, 680.
      Kostoulas, G., et al. 1999. FEBS Lett. 455, 286.
      Strojnik, T., et al. 1999. Clin. Cancer Res. 5, 559.
      Maquire, T.M., et al. 1998. Int. J. Biol. Markers 13, 139.
      Berquim, I.M., and Sloane, B.F. 1996. Adv. Exp. Med. Biol. 389, 281.

      Brochure

      Title
      Cathepsins and Related Products Technical Bulletin
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision18-May-2010 JSW
      DescriptionNative cathepsin B from human liver, purified by affinity chromatography and HPLC. The most investigated enzyme of all lysosomal cysteine proteases. Upregulation and secretion of cathepsin B have been shown to occur in many types of tumors and to correlate positively with their invasive and metastatic capabilities.
      FormLiquid
      FormulationIn 50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0.
      SourcePrepared from tissues of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      CAS number9047-22-7
      EC number3.4.22.1
      Purity≥95% by SDS-PAGE
      Specific activity≥10 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-RR-β-naphthylamide per min at 40°C, using 100 mM Na+/K+ pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      Merck USA index14, 1905
      ReferencesHirai, K., et al. 1999. Hum. Pathol. 30, 680.
      Kostoulas, G., et al. 1999. FEBS Lett. 455, 286.
      Strojnik, T., et al. 1999. Clin. Cancer Res. 5, 559.
      Maquire, T.M., et al. 1998. Int. J. Biol. Markers 13, 139.
      Berquim, I.M., and Sloane, B.F. 1996. Adv. Exp. Med. Biol. 389, 281.