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16-235 Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody, Alexa Fluor® 488

This Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody, Alexa Fluor 488 is validated for use in FC, IC for the detection of phospho-erbB-2/HER-2 (Tyr1248).

Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody, Alexa Fluor® 488 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Growth Factors & Receptors, Kinases & Phosphatases Gene Symbol: ERBB2, HER2, HER-2, NGL, herstatin, erbB-2, C-erbB-2, NEU, TKR1, p185erbB2, HER-2/neu UniProt Number: P04626
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16-235
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RFC, ICCRbAlexaFluor®488Polyclonal Antibody
      Description
      Catalogue Number16-235
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-erbB-2/HER-2 (Tyr1248) Antibody, Alexa Fluor® 488
      Product Information
      FormatAlexaFluor®488
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody, Alexa Fluor 488 is validated for use in FC, IC for the detection of phospho-erbB-2/HER-2 (Tyr1248).
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      Biological Information
      ImmunogenSynthetic phosphopeptide, residues 1243-1255, corresponding to a major autophosphorylation (Tyr1248) site of human Erb B2/HER-2 (C-AENPEpYLGLDVPV)
      HostRabbit
      Specificitythe activated form of Erb B2/HER-2 oncoprotein
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NotePredicted to cross react with rat based on conservation of immunogenic sequence
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
      Gene Symbol
      • ERBB2
      • HER2
      • HER-2
      • NGL
      • herstatin
      • erbB-2
      • C-erbB-2
      • NEU
      • TKR1
      • p185erbB2
      • HER-2/neu
      Modifications
      • Phosphorylation
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04626 # Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF- alpha and amphiregulin.
      SIZE: 1255 amino acids; 137910 Da
      SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with PRKCABP and PLXNB1. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with MEMO when phosphorylated on Tyr-1248.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      PTM: Ligand-binding increases phosphorylation on tyrosine residues (By similarity).
      SIMILARITY: SwissProt: P04626 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      Molecular Weight185kDa
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunostaining of EGF treated A431 cells
      Usage Statement
      • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at 4°C from date of shipment
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      16-235 04053252507663