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07-2268 Anti-phospho-TBC1D1 Antibody (Ser237)

Anti-phospho-TBC1D1 (Ser237), Cat. No. 07-2268, is a rabbit polyclonal antibody that detects TBC1D1 phosphorylated on serine 237 and is tested for use in Peptide Inhibition Assay and Western Blotting.

Anti-phospho-TBC1D1 Antibody (Ser237) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Insulin/Energy Signaling, Glucose/Glycogen Metabolism Gene Symbol: TBC1D1, TBC, TBC1 UniProt Number: Q86TI0
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07-2268
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, PIARbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-2268
      DescriptionAnti-phospho-TBC1D1 Antibody (Ser237)
      Alternate Names
      • TBC1 domain family member 1
      • TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1
      Background InformationTBC1 domain family member 1 (UniProt: Q86TI0; also known as TBC1D1) is encoded by the TBC1D1 gene (Gene ID: 23216) in human. TBC1D1 is a member of the TBC1 Rab-GTPase family of nuclear proteins that is highly expressed in skeletal muscle. Its major action is to regulate exercise-mediated glucose uptake into muscle. It is involved in the trafficking and translocation of GLUT4-containing vesicles and insulin-stimulated glucose uptake into cells. Insulin and muscle contraction are reported to increase TBC1D1 phosphorylation on phospho-Akt substrate motifs. Insulin-stimulated phosphorylation by AKT is shown to stimulate GLUT4 translocation. Following muscle exercise, phosphorylation of TBC1D1 at serine 237 by AMPK and 14-3-3 protein binding capacity are significantly increased. Knockdown of TBC1D1 in C2C12 muscle cells is shown to increase fatty acid oxidation and reduce muscle levels of GLUT4. Although TBC1D1 knockout mice do not exhibit any disruption in their whole-body insulin action, they display impaired exercise-regulated metabolism. A mutation in TBC1D1 (R125W) has been linked to obesity in human. (Ref.: Stockli, J., et al. (2015). Diabetes. 64(6); 1914-1922; An, D., et al. (2010). Diabetes. 59(6); 1358-1365; Frosig, C., et al. (2010). J. Physiol. 588(22); 4539-4548).
      References
      Product Information
      FormatAffinity Purified
      Control
      • Lambda phosphatase treated and untreated HEK293 cell lysates
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-TBC1D1 (Ser237), Cat. No. 07-2268, is a rabbit polyclonal antibody that detects TBC1D1 phosphorylated on serine 237 and is tested for use in Peptide Inhibition Assay and Western Blotting.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Application NotesTested Applications

      Peptide Inhibition Assay: Target band detection in HEK293 cell lysate was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphoserine 237 of human TBC1 domain family member 1 (TBC1D1).
      EpitopePhosphorylated Ser237
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects TBC1D1 phosphorylated on serine 237.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Monkey, Chimpanzee, Mouse, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryTBC1D1 is the founding member of a family of proteins sharing a 180- to 200-amino acid TBC domain presumed to have a role in regulating cell growth and differentiation. These proteins share significant homology with TRE2 (USP6; MIM 604334), yeast Bub2, and CDC16 (MIM 603461) (White et al., 2000 [PubMed 10965142]).[supplied by OMIM].
      Gene Symbol
      • TBC1D1
      • TBC
      • TBC1
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: May act as a GTPase-activating protein for Rab family protein(s). May play a role in the cell cycle and differentiation of various tissues (By similarity).

      SUBCELLULAR LOCATION: Nucleus (By similarity).

      SEQUENCE SIMILARITIES: Contains 1 PID domain.

      Contains 1 Rab-GAP TBC domain.

      SEQUENCE CAUTION: The sequence BAB55057.1 differs from that shown. Reason: Erroneous initiation.
      Molecular Weight~158 kDa observed; 133.08 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from HEK293 cells treated with phorbol-12-myristate-13-acetate
      (PMA).

      Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected TBC1D1 lysate from HEK293 cells treated with phorbol-12-myristate-13-acetate (PMA). However, the detection was inhibited blots pre-incubated with protein phosphatase.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-2268 04053252292149

      Documentation

      Anti-phospho-TBC1D1 Antibody (Ser237) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-TBC1D1 Antibody (Ser237) Certificates of Analysis

      TitleLot Number
      Anti-phospho-TBC1D1 (Ser237) - 2390581 2390581
      Anti-phospho-TBC1D1 (Ser237) - 2427514 2427514
      Anti-phospho-TBC1D1 (Ser237) - 2061452 2061452
      Anti-phospho-TBC1D1 (Ser237) - 2204971 2204971
      Anti-phospho-TBC1D1 (Ser237) - 2273986 2273986
      Anti-phospho-TBC1D1 (Ser237) - 2562374 2562374
      Anti-phospho-TBC1D1 (Ser237) - 3286488 3286488
      Anti-phospho-TBC1D1 (Ser237) - 3393428 3393428
      Anti-phospho-TBC1D1 (Ser237) - 3517960 3517960
      Anti-phospho-TBC1D1 (Ser237) - 3692729 3692729
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