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ABS1515 Anti-phospho NFkB p52, (Ser222) Antibody

Anti-phospho NFkB p52, (Ser222) is an antibody against phospho NFkB p52 for use in Western Blotting.

Anti-phospho NFkB p52, (Ser222) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: NFKB2, LYT10 UniProt Number: Q00653
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ABS1515
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS1515
      DescriptionAnti-phospho NFkB p52, (Ser222) Antibody
      Alternate Names
      • Nuclear factor NF-kappa-B p100 subunit
      • DNA-binding factor KBF2
      • H2TF1
      • Lymphocyte translocation chromosome 10 protein
      • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2
      • Oncogene Lyt-10
      • Lyt10
      • Nuclear factor NF-kappa-B p52 subunit
      Background InformationThe p52 NF-kB subunit is induced through activation of the alternative (noncanonical) NF-kB pathway in response to a range of stimuli such as lymphotoxin-b, BAFF and CD40, as well as DNA damage. The p52 formation results from proteolytic processing of its precursor protein p100 as a consequence of IKKalpha-mediated phosphorylation. The p52 is most commonly found as a heterodimer with RelB, although other dimer combinations have been identified by ChIP analysis. Ser222 of NF-kappa B p52 is phosphorylated by the kinase GSK3beta. Phosphorylation at S222 appears to regulate not only p52 partner dimerization but also the p52 dependent activation or repression of Skp2 gene expression.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho NFkB p52, (Ser222) is an antibody against phospho NFkB p52 for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected phospho NFkB p52, (Ser222) in CD40L and NTL cell lysate (Neil D. Perkins, Newcastle University).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the RHD domain of human phospho NFkB p52, (Ser222).
      EpitopeRHD domain
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NFKB2
      • LYT10
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~52 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in cell lysate U2OS transfected with p52 WT.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho NFkB p52, (Ser222) in 10 µg of cell lysate U2OS transfected with p52 WT.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS1515 04055977127348

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies