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ABF192 Anti-phospho-MDA-5 (Ser104)

Anti-phospho-MDA-5 (Ser104), Cat. No. ABF192 is a rabbit polyclonal antibody that detects MDA-5 phosphorylated on Ser104 and has been tested for use in Western Blotting and Peptide Inhibition Assay.

Anti-phospho-MDA-5 (Ser104) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: IFIH1, MDA5, RH116 UniProt Number: Q9BYX4
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ABF192
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, PIARbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABF192
      DescriptionAnti-phospho-MDA-5 (Ser104)
      Alternate Names
      • Interferon-induced helicase C domain-containing protein 1
      • CADM-140 autoantigen
      • Helicase with 2 CARD domains
      • Helicard
      • Interferon-induced with helicase C domain protein 1
      • Melanoma differentiation-associated protein 5
      • MDA-5
      • Murabutide down-regulated protein
      • RIG-I-like receptor 2
      • RLR-2
      • RNA helicase-DEAD box protein 116
      Background InformationInterferon-induced helicase C domain-containing protein 1 (UniProt: Q9BYX4; also known as EC: 3.6.4.13, Clinically amyopathic dermatomyositis autoantigen 140 kDa, CADM-140 autoantigen, Helicase with 2 CARD domains, Helicard, Interferon-induced with helicase C domain protein 1, Melanoma differentiation-associated protein 5, MDA-5, Murabutide down-regulated protein, RIG-I-like receptor 2, RLR-2, RNA helicase-DEAD box protein 116) is encoded by the IFIH1 (also known as MDA5, RH116) gene (Gene ID: 64135) in human. MDA-5 is an innate immune receptor that acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and pro-inflammatory cytokines. MDA-5 is widely expressed, but at low levels. Expression is detected at slightly higher levels in placenta, pancreas and spleen. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA. In the absence of ligands it is monomeric and homodimerizes in the presence of dsRNA ligands. Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1), which activates the IKK-related kinases TBK1 and IKBKE that phosphorylate interferon regulatory factors IRF3 and IRF7. These factors activate transcription of antiviral immunological genes, including interferons alpha and beta. MDA-5 also plays an important role in enhancing natural killer cell function and is believed to be involved in growth inhibition and apoptosis in several tumor cell lines. MDA-5 contains 2 CARD domains; 1 helicase ATP-binding domain, 1 helicase C-terminal domain, and 1 RLR C-terminal regulatory domain.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4 with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-MDA-5 (Ser104), Cat. No. ABF192 is a rabbit polyclonal antibody that detects MDA-5 phosphorylated on Ser104 and has been tested for use in Western Blotting and Peptide Inhibition Assay.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Application NotesPeptide Inhibition Analysis: A 1:500 dilution from a representative lot detected phospho-MDA-5 (Ser104) in Raji cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 12 amino acids surrounding Ser104 from the N-terminal region of human MDA-5.
      EpitopeN-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects human MDA-5 protein phosphorylated on Ser104.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IFIH1
      • MDA5
      • RH116
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~117 kDa observed; 116.69 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Daudi cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-MDA-5 (Ser104) in 10 µg of Daudi cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABF192 04054839316005