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MABT165 Anti-p130cas/BCAR1 Antibody, clone 2C1.1

Anti-p130cas/BCAR1 Antibody, clone 2C1.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeletal Signaling Gene Symbol: BCAR1, CAS, CAS1, CASS1, CRKAS UniProt Number: P56945
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MABT165
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT165
      DescriptionAnti-p130cas/BCAR1 Antibody, clone 2C1.1
      Alternate Names
      • Breast cancer anti-estrogen resistance protein 1
      • Cas scaffolding protein family member 1
      • Crk-associated substrate
      • p130Cas
      Background InformationBreast cancer anti-estrogen resistance protein 1 (UniProt P56945; also known as Cas scaffolding protein family member 1, Crk-associated substrate, p130Cas) is encoded by the BCAR1 (also known as CAS, CAS1, CASS1, CRKAS) gene (Gene ID 9564) in human. p130Cas/BCAR1 is an adaptor protein that belongs to the CAS family of scaffold proteins, members of which also include Nedd9, HEF-1 (CAs-L), EFS, and CASS4. Structurally, p130Cas is composed of a Src-homology 3 (SH3) domain, an adjacent large substrate-binding domain containing 15 repetitions of the YxxP motif, a main site of tyrosine phosphorylation on the p130Cas/BCAR1 molecule that generates SH2-binding sites, a proline and serine rich region, and a highly conserved four-helix bundle (focal adhesion targeting [FAT] domain). p130Cas/BCAR1 plays an important role in transducing signaling originated from mechanical stimuli, extracellular matrix (ECM) adhesion, as well as growth factor stimulation cascades during normal development and diseases.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgMκ antibody in PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged recombinant human p130cas/BCAR1 C-terminal fragment.
      EpitopeC-terminal region.
      Clone2C1.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityExpected to react with all eight spliced isoforms of human p130Cas reported by UniProt (P56945-1 through P56945-8).
      IsotypeIgMκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BCAR1
      • CAS
      • CAS1
      • CASS1
      • CRKAS
      UniProt Number
      Molecular Weight~93 kDa observed. 93.14-97.88 kDa (isoforms 1-3 and isoforms 5-8) and 77.73 kDa (isoform 4) calculated. Fully phosphorylated target band migrates with an apparent molecular size of ~130 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected p130cas/BCAR1 in 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT165 04055977311747