Millipore Sigma Vibrant Logo

ABN1477 Anti-nSR100 (SRRM4)

Anti-nSR100 (SRRM4), Cat. No. ABN1477, is a highly specific rabbit polyclonal antibody that targets Serine/arginine repetitive matrix protein 4 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-nSR100 (SRRM4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: SRRM4, KIAA1853 UniProt Number: A7MD48
View This Product on Sigma-Aldrich
ABN1477
100 μg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWB, IH(P)RbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN1477
      DescriptionAnti-nSR100 (SRRM4)
      Alternate Names
      • Serine/arginine repetitive matrix protein 4
      • Medulloblastoma antigen MU-MB-2.76
      • Neural-specific serine/arginine repetitive splicing factor of 100 kDa
      • Neural-specific SR-related protein of 100 kDa
      • nSR100
      Background InformationSerine/arginine repetitive matrix protein 4 (UniProt: A7MD48; also known as Medulloblastoma antigen MU-MB-2.76, Neural-specific serine/arginine repetitive splicing factor of 100 kDa, Neural-specific SR-related protein of 100 kDa, nSR100) is encoded by the SRRM4 (also known as KIAA1853) gene (Gene ID: 84530) in human. nSR100 is a nuclear protein that is specifically expressed in neuronal cells. It serves as a splicing factor that is specifically required for neural cell differentiation. It acts in conjunction with nPTB/PTBP2 by binding directly to its regulated target transcripts and promote neural-specific exon inclusion in many genes that function in neural cell differentiation. It is shown to regulate the splicing of protrudin gene (Zfyve27) transcripts in neuronal cells and promotes splicing of protrudin pre-mRNA so as to include a microexon (exon L) encoding seven amino acids in a neuron-specific manner. The resulting protein known as Protrudin-L promotes neurite outgrowth during neurogenesis. Depletion of nSR100 in Neuro2A cells is shown to impair neurite outgrowth, whereas its ablation in mouse embryos is reported to prevent differentiation of neuronal progenitors in the cortex and impair development of the central and peripheral nervous systems. (Ref.: Ohnishi, T et al. (2017). Sci. Rep. 7: 41130; Quesnel-Vallières, M et al. (2015). Genes Dev. 29(7); 746-759).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-nSR100 (SRRM4), Cat. No. ABN1477, is a highly specific rabbit polyclonal antibody that targets Serine/arginine repetitive matrix protein 4 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected nSR100 (SRRM4) in mouse cerebral cortex and human cerebral cortex tissue sections.
      Biological Information
      ImmunogenGST/His-tagged recombinant frgment corresponding to 104 amino acids from the C-terminal half of human Serine/arginine repetitive matrix protein 4.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Serine/arginine repetitive matrix protein 4 in human and mouse cells. It targets an epitope within 104 amino acids from the C-terminal half.
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SRRM4
      • KIAA1853
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~68 kDa observed; 68.56 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human cerebellum tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected nSR100 (SRRM4) in 10 µg of human cerebellum tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN1477 04054839399718