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07-2184 Anti-acetyl-GAPDH (Lys160) Antibody

This Anti-acetyl-GAPDH (Lys160) antibody is validated for use in western blotting, peptide inhibition assay & IP for the detection of acetyl-GAPDH (Lys160).

Anti-acetyl-GAPDH (Lys160) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Metabolism Research Sub-Category: Insulin/Energy Signaling Gene Symbol: GAPDH, GAPD UniProt Number: P04406
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07-2184
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, PIA, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-2184
      DescriptionAnti-acetyl-GAPDH (Lys160) Antibody
      Alternate Names
      • Glyceraldehyde-3-phosphate dehydrogenase
      • GAPDH
      • Peptidyl-cysteine S-nitrosylase GAPDH
      Background InformationAcetyl-GAPDH (Lys160), also known as Glyceraldehyde-3-phosphate dehydrogenase (GAPDH(lys160), or Peptidyl-cysteine S-nitrosylase GAPDH, and encoded by the gene GAPDH/GAPD, is an interesting multifunctional metabolic enzyme that plays a critical role in glycolysis but also many other important nuclear and cell functions such as transcription, RNA transport, DNA replication and even apoptosis and cell death. In glycolysis, GAPDH is a key enzyme that catalyzes the first step in the pathway by converting G3P into 3-phospho-D glyceroyl phosphate. An example of GAPDH’s regulation of gene transcription as a component of a GAIT complex, which selectively inhibits the activation of various gamma interferon induced inflammation responses. The protein-protein interactions of GAPDH are at least partially controlled by acetylation. For instance, transport of GAPDH into the nucleus is dependent upon acetylation at lysines 117, 227, and 251, and acetylation at lysine 183 governs co-factor interactions. Acetylation of GAPDH at lysine 160 ultimately is involved in p53 mediated cell death.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-acetyl-GAPDH (Lys160) antibody is validated for use in western blotting, peptide inhibition assay & IP for the detection of acetyl-GAPDH (Lys160).
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      • Immunoprecipitation
      Application NotesPeptide Inhibition Analysis: 1 µg/mL from a representative lot detected acetyl GAPDH (Lys60) in LPS/IFNgamma in RAW264.7 nuclear extract.

      Immunoprecipitation Analyis: A representative lot immunoprecipitated acetyl GAPDH (Lys60) from LPS/IFNgamma treated RAW264.7 cell lysate (Prof. Akira Sawa, Johns Hopkins University, Baltimore.).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the human GAPDH acetylated at Lys160.
      Epitope0
      Concentration0.5 mg/mL
      HostRabbit
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Rat, Non-Human Primate, Bovine, Horse, Feline, Sheep, Porcine, and Canine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GAPDH
      • GAPD
      Modifications
      • Acetylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~37 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in untreated and LPS/IFNgamma treated RAW264.7 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected acetyl GAPDH (Lys60) in LPS/IFNgamma treated RAW264.7 cell lysate and not in untreated RAW264.7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-2184 04053252971167

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies